Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats
Samo za registrovane korisnike
2019
Autori
Đedović, Neda![](/themes/Mirageimagine/images/orcid.png)
Jevtić, Bojan
![](/themes/Mirageimagine/images/orcid.png)
Jose Mansilla, M.
Petković, Filip
![](/themes/Mirageimagine/images/orcid.png)
Blazevski, Jana
Timotijević, Gordana
![](/themes/Mirageimagine/images/orcid.png)
Navarro-Barriuso, Juan
![](/themes/Mirageimagine/images/orcid.png)
Martinez-Caceres, Eva
![](/themes/Mirageimagine/images/orcid.png)
Mostarica Stojkovide, Marija
Miljković, Đorđe
![](/themes/Mirageimagine/images/orcid.png)
Članak u časopisu (Objavljena verzija)
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Dendritic cells (DC) are responsible for the initiation and shaping of the adaptive immune response and are in the focus of autoimmunity research. We were interested in comparison of DC obtained from autoimmunity-prone Dark Agouti (DA) rats and autoimmunity-resistant Albino Oxford (AO) rats. DC were generated from bone marrow precursors and matured (mDC) by lipopolysaccharide. Tolerogenic DC (tolDC) obtained by vitamin D3 treatment were studied in parallel. Profile of cytokine production was different in AO and DA mDC and tolDC. Expression of MHC class II molecules and CD86 were higher in DA DC, while vitamin D3 reduced their expression in dendritic cells of both strains. Allogeneic proliferation of CD4(+) T cells was reduced by AO tolDC, but not with DA tolDC in comparison to respective mDC. Finally, expression of various genes identified as differentially expressed in human mDC and tolDC was also analyzed in AO and DA DC. Again, AO and DA DC differed in the expression of the analyzed... genes. To conclude, AO and DA DC differ in production of cytokines, expression of antigen presentation-related molecules and in regulation of CD4(+) T proliferation. The difference is valuable for understanding the divergence of the strains in their susceptibility to autoimmunity.
Ključne reči:
Dendritic cells / Dark agouti rats / Autoimmunity / Allogeneic proliferation / Albino Oxford ratsIzvor:
Immunobiology, 2019, 224, 3, 470-476Izdavač:
- Elsevier Gmbh, Munich
Finansiranje / projekti:
- Plan Nacional de I + D + I [PI14/01175, PI17/01521]
- ISCIII-Subdireccion General de Evaluaci6n
- Fondo Europeo de Desarrollo Regional (FEDER)
- Short Term Scientific Missions through A FACTT network (Cost Action) [BM1305]
- EU Framework Programme Horizon 2020
- Ćelijski i molekulski mehanizmi oporavka pacova od eksperimentalnog autoimunskog encefalomijelitisa (RS-MESTD-Basic Research (BR or ON)-173035)
- Molekularni mehanizmi fiziološke i farmakološke kontrole inflamacije i kancera (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.imbio.2019.01.001
ISSN: 0171-2985
PubMed: 30765133
WoS: 000473836000019
Scopus: 2-s2.0-85061255964
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Đedović, Neda AU - Jevtić, Bojan AU - Jose Mansilla, M. AU - Petković, Filip AU - Blazevski, Jana AU - Timotijević, Gordana AU - Navarro-Barriuso, Juan AU - Martinez-Caceres, Eva AU - Mostarica Stojkovide, Marija AU - Miljković, Đorđe PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1252 AB - Dendritic cells (DC) are responsible for the initiation and shaping of the adaptive immune response and are in the focus of autoimmunity research. We were interested in comparison of DC obtained from autoimmunity-prone Dark Agouti (DA) rats and autoimmunity-resistant Albino Oxford (AO) rats. DC were generated from bone marrow precursors and matured (mDC) by lipopolysaccharide. Tolerogenic DC (tolDC) obtained by vitamin D3 treatment were studied in parallel. Profile of cytokine production was different in AO and DA mDC and tolDC. Expression of MHC class II molecules and CD86 were higher in DA DC, while vitamin D3 reduced their expression in dendritic cells of both strains. Allogeneic proliferation of CD4(+) T cells was reduced by AO tolDC, but not with DA tolDC in comparison to respective mDC. Finally, expression of various genes identified as differentially expressed in human mDC and tolDC was also analyzed in AO and DA DC. Again, AO and DA DC differed in the expression of the analyzed genes. To conclude, AO and DA DC differ in production of cytokines, expression of antigen presentation-related molecules and in regulation of CD4(+) T proliferation. The difference is valuable for understanding the divergence of the strains in their susceptibility to autoimmunity. PB - Elsevier Gmbh, Munich T2 - Immunobiology T1 - Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats EP - 476 IS - 3 SP - 470 VL - 224 DO - 10.1016/j.imbio.2019.01.001 ER -
@article{ author = "Đedović, Neda and Jevtić, Bojan and Jose Mansilla, M. and Petković, Filip and Blazevski, Jana and Timotijević, Gordana and Navarro-Barriuso, Juan and Martinez-Caceres, Eva and Mostarica Stojkovide, Marija and Miljković, Đorđe", year = "2019", abstract = "Dendritic cells (DC) are responsible for the initiation and shaping of the adaptive immune response and are in the focus of autoimmunity research. We were interested in comparison of DC obtained from autoimmunity-prone Dark Agouti (DA) rats and autoimmunity-resistant Albino Oxford (AO) rats. DC were generated from bone marrow precursors and matured (mDC) by lipopolysaccharide. Tolerogenic DC (tolDC) obtained by vitamin D3 treatment were studied in parallel. Profile of cytokine production was different in AO and DA mDC and tolDC. Expression of MHC class II molecules and CD86 were higher in DA DC, while vitamin D3 reduced their expression in dendritic cells of both strains. Allogeneic proliferation of CD4(+) T cells was reduced by AO tolDC, but not with DA tolDC in comparison to respective mDC. Finally, expression of various genes identified as differentially expressed in human mDC and tolDC was also analyzed in AO and DA DC. Again, AO and DA DC differed in the expression of the analyzed genes. To conclude, AO and DA DC differ in production of cytokines, expression of antigen presentation-related molecules and in regulation of CD4(+) T proliferation. The difference is valuable for understanding the divergence of the strains in their susceptibility to autoimmunity.", publisher = "Elsevier Gmbh, Munich", journal = "Immunobiology", title = "Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats", pages = "476-470", number = "3", volume = "224", doi = "10.1016/j.imbio.2019.01.001" }
Đedović, N., Jevtić, B., Jose Mansilla, M., Petković, F., Blazevski, J., Timotijević, G., Navarro-Barriuso, J., Martinez-Caceres, E., Mostarica Stojkovide, M.,& Miljković, Đ.. (2019). Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats. in Immunobiology Elsevier Gmbh, Munich., 224(3), 470-476. https://doi.org/10.1016/j.imbio.2019.01.001
Đedović N, Jevtić B, Jose Mansilla M, Petković F, Blazevski J, Timotijević G, Navarro-Barriuso J, Martinez-Caceres E, Mostarica Stojkovide M, Miljković Đ. Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats. in Immunobiology. 2019;224(3):470-476. doi:10.1016/j.imbio.2019.01.001 .
Đedović, Neda, Jevtić, Bojan, Jose Mansilla, M., Petković, Filip, Blazevski, Jana, Timotijević, Gordana, Navarro-Barriuso, Juan, Martinez-Caceres, Eva, Mostarica Stojkovide, Marija, Miljković, Đorđe, "Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats" in Immunobiology, 224, no. 3 (2019):470-476, https://doi.org/10.1016/j.imbio.2019.01.001 . .