Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro
Аутори
Popović, JelenaLazić, Andrijana
Paunesku, Tatjana
Ma, Qing
Chen, Si
Lai, Barry
Stevanović, Milena
Woloschak, Gayle E.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ra...y absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN.
Кључне речи:
XFM / Neuroprotection / Cisplatin / CIPN / AmifostineИзвор:
Cellular and Molecular Neurobiology, 2019, 39, 5, 619-636Издавач:
- Springer/Plenum Publishers, New York
Финансирање / пројекти:
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
- UICC Yamagiwa-Yoshida Memorial International Cancer Study Grant [YY2/2015/381414]
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-06CH11357]
- NIH ARRA Grant [SP0007167]
- Northwestern University
- E. I. DuPont de Nemours Co.
- Dow Chemical Company
- NCI CCSG [P30 CA060553]
- NIH [1S10OD010398-01]
DOI: 10.1007/s10571-019-00667-7
ISSN: 0272-4340
PubMed: 30874981
WoS: 000469033100005
Scopus: 2-s2.0-85062973382
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Popović, Jelena AU - Lazić, Andrijana AU - Paunesku, Tatjana AU - Ma, Qing AU - Chen, Si AU - Lai, Barry AU - Stevanović, Milena AU - Woloschak, Gayle E. PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1255 AB - Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ray absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN. PB - Springer/Plenum Publishers, New York T2 - Cellular and Molecular Neurobiology T1 - Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro EP - 636 IS - 5 SP - 619 VL - 39 DO - 10.1007/s10571-019-00667-7 ER -
@article{ author = "Popović, Jelena and Lazić, Andrijana and Paunesku, Tatjana and Ma, Qing and Chen, Si and Lai, Barry and Stevanović, Milena and Woloschak, Gayle E.", year = "2019", abstract = "Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ray absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN.", publisher = "Springer/Plenum Publishers, New York", journal = "Cellular and Molecular Neurobiology", title = "Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro", pages = "636-619", number = "5", volume = "39", doi = "10.1007/s10571-019-00667-7" }
Popović, J., Lazić, A., Paunesku, T., Ma, Q., Chen, S., Lai, B., Stevanović, M.,& Woloschak, G. E.. (2019). Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro. in Cellular and Molecular Neurobiology Springer/Plenum Publishers, New York., 39(5), 619-636. https://doi.org/10.1007/s10571-019-00667-7
Popović J, Lazić A, Paunesku T, Ma Q, Chen S, Lai B, Stevanović M, Woloschak GE. Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro. in Cellular and Molecular Neurobiology. 2019;39(5):619-636. doi:10.1007/s10571-019-00667-7 .
Popović, Jelena, Lazić, Andrijana, Paunesku, Tatjana, Ma, Qing, Chen, Si, Lai, Barry, Stevanović, Milena, Woloschak, Gayle E., "Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro" in Cellular and Molecular Neurobiology, 39, no. 5 (2019):619-636, https://doi.org/10.1007/s10571-019-00667-7 . .