In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release
Nema prikaza
Autori
Filipović, Vuk V.Babić, Marija M.
Godevac, Dejan
Pavić, Aleksandar
Nikodinović-Runić, Jasmina
Tomić, Simonida Lj
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and the...ir degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.
Ključne reči:
poly-beta amino esters zwitterionic hydrogels / pH-sensitive / drug delivery / degradable / biocompatibleIzvor:
Macromolecular Chemistry and Physics, 2019, 220, 17Izdavač:
- Wiley-V C H Verlag Gmbh, Weinheim
Finansiranje / projekti:
- Sinteza i karakterizacija novih funkcionalnih polimera i polimernih nanokompozita (RS-MESTD-Basic Research (BR or ON)-172062)
- Hemijsko i strukturno dizajniranje nanomaterijala za primenu u medicini i inženjerstvu tkiva (RS-MESTD-Basic Research (BR or ON)-172026)
DOI: 10.1002/macp.201900188
ISSN: 1022-1352
WoS: 000480503900001
Scopus: 2-s2.0-85070695892
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Filipović, Vuk V. AU - Babić, Marija M. AU - Godevac, Dejan AU - Pavić, Aleksandar AU - Nikodinović-Runić, Jasmina AU - Tomić, Simonida Lj PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1256 AB - New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems. PB - Wiley-V C H Verlag Gmbh, Weinheim T2 - Macromolecular Chemistry and Physics T1 - In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release IS - 17 VL - 220 DO - 10.1002/macp.201900188 ER -
@article{ author = "Filipović, Vuk V. and Babić, Marija M. and Godevac, Dejan and Pavić, Aleksandar and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj", year = "2019", abstract = "New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.", publisher = "Wiley-V C H Verlag Gmbh, Weinheim", journal = "Macromolecular Chemistry and Physics", title = "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release", number = "17", volume = "220", doi = "10.1002/macp.201900188" }
Filipović, V. V., Babić, M. M., Godevac, D., Pavić, A., Nikodinović-Runić, J.,& Tomić, S. L.. (2019). In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics Wiley-V C H Verlag Gmbh, Weinheim., 220(17). https://doi.org/10.1002/macp.201900188
Filipović VV, Babić MM, Godevac D, Pavić A, Nikodinović-Runić J, Tomić SL. In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics. 2019;220(17). doi:10.1002/macp.201900188 .
Filipović, Vuk V., Babić, Marija M., Godevac, Dejan, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj, "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release" in Macromolecular Chemistry and Physics, 220, no. 17 (2019), https://doi.org/10.1002/macp.201900188 . .