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Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus
dc.creator | Jeremić, Ivica | |
dc.creator | Đurić, Olivera | |
dc.creator | Nikolić, Milos | |
dc.creator | Vlajnić, Marina | |
dc.creator | Nikolić, Aleksandra | |
dc.creator | Radojković, Dragica | |
dc.creator | Bonaci-Nikolić, Branka | |
dc.date.accessioned | 2022-11-15T15:07:32Z | |
dc.date.available | 2022-11-15T15:07:32Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0172-8172 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/1257 | |
dc.description.abstract | Neutrophil extracellular traps (NETs) are the main source of autoantigens in systemic lupus erythematosus (SLE). The aim of this study was to evaluate the clinical importance of NETs-associated markers in SLE. We compared NETs-associated markers in SLE patients (n = 111) with healthy controls (n = 50). Moreover, in 35 patients with drug-naive SLE (n = 35), we investigated correlation between NETs-associated markers [DNase I concentration, myeloperoxidase (MPO) activity, anti-MPO antibodies, cell-free DNA (cfDNA), NETolytic activity] with serological parameters [anti-dsDNA antibodies, C3, C4 and B-cell activating factor (BAFF) levels] and disease activity measured by modified SLE Disease Activity Index (M-SLEDAI-2K). In comparison with healthy controls, SLE patients had higher cfDNA, MPO activity, anti-MPO antibodies (p lt 0.001), BAFF and DNase I concentration (p lt 0.01). Contrary, NETolytic activity was lower in SLE patients (p lt 0.05), despite higher concentration of DNase I. MPO activity and cfDNA levels showed correlation with DNase I concentration (p lt 0.001, p lt 0.01, respectively). BAFF levels correlated with cfDNA, DNase I concentration and MPO activity (p lt 0.05). Anti-dsDNA antibodies showed correlation with MPO activity (p lt 0.01), cfDNA and BAFF levels (p lt 0.001). Anti-dsDNA and C3 levels were independent predictors of M-SLEDAI-2K in multivariate analysis (p lt 0.01). We demonstrated that sera of SLE patients have decreased NETolytic activity, leading to increased levels of various NETs-associated markers, which correlate with anti-dsDNA antibodies in drug-naive SLE. We showed that BAFF participates in a complex relationship between NETosis and anti-dsDNA antibodies production. These findings have important implications for a better understanding of SLE pathogenesis and development of therapy that inhibits NETs persistence and disease progression. | en |
dc.publisher | Springer Heidelberg, Heidelberg | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175065/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173008/RS// | |
dc.rights | restrictedAccess | |
dc.source | Rheumatology International | |
dc.subject | SLE | en |
dc.subject | NET | en |
dc.subject | DNase I | en |
dc.subject | Cell-free DNA | en |
dc.subject | BAFF | en |
dc.subject | Anti-dsDNA | en |
dc.title | Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1857 | |
dc.citation.issue | 11 | |
dc.citation.other | 39(11): 1849-1857 | |
dc.citation.rank | M23 | |
dc.citation.spage | 1849 | |
dc.citation.volume | 39 | |
dc.identifier.doi | 10.1007/s00296-019-04426-1 | |
dc.identifier.pmid | 31444555 | |
dc.identifier.scopus | 2-s2.0-85070999809 | |
dc.identifier.wos | 000490008100002 | |
dc.type.version | publishedVersion |