Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns
Authors
Zavisić, Gordana N.Petricević, Sasa M.
Ristić, Slavica M.
Rikalović, Milena G.
Jovanović-Ljesković, Nataša M.
Begović, Jelena
Strahinić, Ivana
Article (Published version)
Metadata
Show full item recordAbstract
The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. ente...rica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.
Keywords:
safety / probiotic / meconium / Lactobacillus fermentumSource:
Journal of the Serbian Chemical Society, 2019, 84, 4, 365-376Publisher:
- Srpsko hemijsko društvo, Beograd
Funding / projects:
- Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.2298/JSC181105015Z
ISSN: 0352-5139
WoS: 000466010400003
Scopus: 2-s2.0-85069298820
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Zavisić, Gordana N. AU - Petricević, Sasa M. AU - Ristić, Slavica M. AU - Rikalović, Milena G. AU - Jovanović-Ljesković, Nataša M. AU - Begović, Jelena AU - Strahinić, Ivana PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1268 AB - The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns EP - 376 IS - 4 SP - 365 VL - 84 DO - 10.2298/JSC181105015Z ER -
@article{ author = "Zavisić, Gordana N. and Petricević, Sasa M. and Ristić, Slavica M. and Rikalović, Milena G. and Jovanović-Ljesković, Nataša M. and Begović, Jelena and Strahinić, Ivana", year = "2019", abstract = "The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns", pages = "376-365", number = "4", volume = "84", doi = "10.2298/JSC181105015Z" }
Zavisić, G. N., Petricević, S. M., Ristić, S. M., Rikalović, M. G., Jovanović-Ljesković, N. M., Begović, J.,& Strahinić, I.. (2019). Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 84(4), 365-376. https://doi.org/10.2298/JSC181105015Z
Zavisić GN, Petricević SM, Ristić SM, Rikalović MG, Jovanović-Ljesković NM, Begović J, Strahinić I. Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society. 2019;84(4):365-376. doi:10.2298/JSC181105015Z .
Zavisić, Gordana N., Petricević, Sasa M., Ristić, Slavica M., Rikalović, Milena G., Jovanović-Ljesković, Nataša M., Begović, Jelena, Strahinić, Ivana, "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns" in Journal of the Serbian Chemical Society, 84, no. 4 (2019):365-376, https://doi.org/10.2298/JSC181105015Z . .