Cloning and characterisation of the rpoS gene from plant growth-promoting Pseudomonas putida WCS358: RpoS is not involved in siderophore and homoserine lactone production
Само за регистроване кориснике
1999
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The rpoS gene which encodes a stationary phase sigma factor has been identified and characterised from the rhizosphere-colonising plant growth-promoting Pseudomonas putida strain WCS358. The predicted protein sequence has extensive homologies with the RpoS proteins form other bacteria, in particular with the RpoS sigma factors of the fluorescent pseudomonads. A genomic transposon insertion in the rpoS gene was constructed, these mutants were analysed for their ability to produce siderophore (iron-transport agent) and the autoinducer quorum-sensing molecules called homoserine lactones (AHL). It was determined that RpoS was not involved in the regulation of siderophore and AHL production, synthesis of these molecules is important for gene expression at stationary phase. P. putida WCS358 produces at least three different AHL molecules.
Кључне речи:
siderophore / RpoS / regulation / quorum sensing / Pseudomonas / iron / homoserine lactoneИзвор:
Biochimica Et Biophysica Acta-Gene Structure and Expression, 1999, 1489, 2-3, 413-420Издавач:
- Elsevier, Amsterdam
DOI: 10.1016/S0167-4781(99)00210-9
ISSN: 0167-4781
PubMed: 10673044
WoS: 000084528300021
Scopus: 2-s2.0-0032755836
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milan AU - Degrassi, G AU - Venturi, V PY - 1999 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/127 AB - The rpoS gene which encodes a stationary phase sigma factor has been identified and characterised from the rhizosphere-colonising plant growth-promoting Pseudomonas putida strain WCS358. The predicted protein sequence has extensive homologies with the RpoS proteins form other bacteria, in particular with the RpoS sigma factors of the fluorescent pseudomonads. A genomic transposon insertion in the rpoS gene was constructed, these mutants were analysed for their ability to produce siderophore (iron-transport agent) and the autoinducer quorum-sensing molecules called homoserine lactones (AHL). It was determined that RpoS was not involved in the regulation of siderophore and AHL production, synthesis of these molecules is important for gene expression at stationary phase. P. putida WCS358 produces at least three different AHL molecules. PB - Elsevier, Amsterdam T2 - Biochimica Et Biophysica Acta-Gene Structure and Expression T1 - Cloning and characterisation of the rpoS gene from plant growth-promoting Pseudomonas putida WCS358: RpoS is not involved in siderophore and homoserine lactone production EP - 420 IS - 2-3 SP - 413 VL - 1489 DO - 10.1016/S0167-4781(99)00210-9 ER -
@article{ author = "Kojić, Milan and Degrassi, G and Venturi, V", year = "1999", abstract = "The rpoS gene which encodes a stationary phase sigma factor has been identified and characterised from the rhizosphere-colonising plant growth-promoting Pseudomonas putida strain WCS358. The predicted protein sequence has extensive homologies with the RpoS proteins form other bacteria, in particular with the RpoS sigma factors of the fluorescent pseudomonads. A genomic transposon insertion in the rpoS gene was constructed, these mutants were analysed for their ability to produce siderophore (iron-transport agent) and the autoinducer quorum-sensing molecules called homoserine lactones (AHL). It was determined that RpoS was not involved in the regulation of siderophore and AHL production, synthesis of these molecules is important for gene expression at stationary phase. P. putida WCS358 produces at least three different AHL molecules.", publisher = "Elsevier, Amsterdam", journal = "Biochimica Et Biophysica Acta-Gene Structure and Expression", title = "Cloning and characterisation of the rpoS gene from plant growth-promoting Pseudomonas putida WCS358: RpoS is not involved in siderophore and homoserine lactone production", pages = "420-413", number = "2-3", volume = "1489", doi = "10.1016/S0167-4781(99)00210-9" }
Kojić, M., Degrassi, G.,& Venturi, V.. (1999). Cloning and characterisation of the rpoS gene from plant growth-promoting Pseudomonas putida WCS358: RpoS is not involved in siderophore and homoserine lactone production. in Biochimica Et Biophysica Acta-Gene Structure and Expression Elsevier, Amsterdam., 1489(2-3), 413-420. https://doi.org/10.1016/S0167-4781(99)00210-9
Kojić M, Degrassi G, Venturi V. Cloning and characterisation of the rpoS gene from plant growth-promoting Pseudomonas putida WCS358: RpoS is not involved in siderophore and homoserine lactone production. in Biochimica Et Biophysica Acta-Gene Structure and Expression. 1999;1489(2-3):413-420. doi:10.1016/S0167-4781(99)00210-9 .
Kojić, Milan, Degrassi, G, Venturi, V, "Cloning and characterisation of the rpoS gene from plant growth-promoting Pseudomonas putida WCS358: RpoS is not involved in siderophore and homoserine lactone production" in Biochimica Et Biophysica Acta-Gene Structure and Expression, 1489, no. 2-3 (1999):413-420, https://doi.org/10.1016/S0167-4781(99)00210-9 . .