Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
Нема приказа
Аутори
Aleksić, MilenaStanisavljević Ninković, Danijela
Smiljković, Marija
Vasiljević, Perica
Stevanović, Milena
Soković, Marina
Stojković, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Pyrimethanil, a synthetic fungicide widely used for the treatment of pre- and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 mu g mu L-1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyri...methanil on human cell lines were recorded already at 50 ng mu L-1, while the expression of Ki67 marker of proliferation was decreased with 150 ng mu L-1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.
Кључне речи:
tomato / pyrimethanil / cytotoxicity / cell proliferation / Aspergillus rot / antifungalИзвор:
Annals of Applied Biology, 2019, 175, 2, 228-235Издавач:
- Wiley, Hoboken
DOI: 10.1111/aab.12532
ISSN: 0003-4746
WoS: 000478613200010
Scopus: 2-s2.0-85069914835
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Aleksić, Milena AU - Stanisavljević Ninković, Danijela AU - Smiljković, Marija AU - Vasiljević, Perica AU - Stevanović, Milena AU - Soković, Marina AU - Stojković, Dejan PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1283 AB - Pyrimethanil, a synthetic fungicide widely used for the treatment of pre- and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 mu g mu L-1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng mu L-1, while the expression of Ki67 marker of proliferation was decreased with 150 ng mu L-1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity. PB - Wiley, Hoboken T2 - Annals of Applied Biology T1 - Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines EP - 235 IS - 2 SP - 228 VL - 175 DO - 10.1111/aab.12532 ER -
@article{ author = "Aleksić, Milena and Stanisavljević Ninković, Danijela and Smiljković, Marija and Vasiljević, Perica and Stevanović, Milena and Soković, Marina and Stojković, Dejan", year = "2019", abstract = "Pyrimethanil, a synthetic fungicide widely used for the treatment of pre- and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 mu g mu L-1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng mu L-1, while the expression of Ki67 marker of proliferation was decreased with 150 ng mu L-1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.", publisher = "Wiley, Hoboken", journal = "Annals of Applied Biology", title = "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines", pages = "235-228", number = "2", volume = "175", doi = "10.1111/aab.12532" }
Aleksić, M., Stanisavljević Ninković, D., Smiljković, M., Vasiljević, P., Stevanović, M., Soković, M.,& Stojković, D.. (2019). Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology Wiley, Hoboken., 175(2), 228-235. https://doi.org/10.1111/aab.12532
Aleksić M, Stanisavljević Ninković D, Smiljković M, Vasiljević P, Stevanović M, Soković M, Stojković D. Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines. in Annals of Applied Biology. 2019;175(2):228-235. doi:10.1111/aab.12532 .
Aleksić, Milena, Stanisavljević Ninković, Danijela, Smiljković, Marija, Vasiljević, Perica, Stevanović, Milena, Soković, Marina, Stojković, Dejan, "Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines" in Annals of Applied Biology, 175, no. 2 (2019):228-235, https://doi.org/10.1111/aab.12532 . .