Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes
Аутори
Mandić, MinaĐokić, Lidija
Nikolaivits, Efstratios
Prodanović, Radivoje
O'Connor, Kevin
Jeremić, Sanja
Topakas, Evangelos
Nikodinović-Runić, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range ...and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes.
Кључне речи:
Pseudomonas / laccase / heterologous expression / genome-mining / biocatalysisИзвор:
Catalysts, 2019, 9, 7Издавач:
- MDPI, Basel
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
- FEMS Research Grant [FEMS-RG-2016-0088]
DOI: 10.3390/catal9070629
ISSN: 2073-4344
WoS: 000478652600029
Scopus: 2-s2.0-85070946373
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mandić, Mina AU - Đokić, Lidija AU - Nikolaivits, Efstratios AU - Prodanović, Radivoje AU - O'Connor, Kevin AU - Jeremić, Sanja AU - Topakas, Evangelos AU - Nikodinović-Runić, Jasmina PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1284 AB - Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes. PB - MDPI, Basel T2 - Catalysts T1 - Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes IS - 7 VL - 9 DO - 10.3390/catal9070629 ER -
@article{ author = "Mandić, Mina and Đokić, Lidija and Nikolaivits, Efstratios and Prodanović, Radivoje and O'Connor, Kevin and Jeremić, Sanja and Topakas, Evangelos and Nikodinović-Runić, Jasmina", year = "2019", abstract = "Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes.", publisher = "MDPI, Basel", journal = "Catalysts", title = "Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes", number = "7", volume = "9", doi = "10.3390/catal9070629" }
Mandić, M., Đokić, L., Nikolaivits, E., Prodanović, R., O'Connor, K., Jeremić, S., Topakas, E.,& Nikodinović-Runić, J.. (2019). Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes. in Catalysts MDPI, Basel., 9(7). https://doi.org/10.3390/catal9070629
Mandić M, Đokić L, Nikolaivits E, Prodanović R, O'Connor K, Jeremić S, Topakas E, Nikodinović-Runić J. Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes. in Catalysts. 2019;9(7). doi:10.3390/catal9070629 .
Mandić, Mina, Đokić, Lidija, Nikolaivits, Efstratios, Prodanović, Radivoje, O'Connor, Kevin, Jeremić, Sanja, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes" in Catalysts, 9, no. 7 (2019), https://doi.org/10.3390/catal9070629 . .