Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles
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Filipović, NenadVeselinović, Ljiljana
Razić, Slavica
Jeremić, Sanja
Filipić, Metka
Zegura, Bojana
Tomić, Sergej
Čolić, Miodrag
Stevanović, Magdalena
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Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while... in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.
Keywords:
Selenium nanoparticles / Prolonged release / PCL / Microspheres / BiodegradationSource:
Materials Science & Engineering C-Materials For Biological Applications, 2019, 96, 776-789Publisher:
- Elsevier, Amsterdam
Funding / projects:
- European Science Foundation COST Action [CA15114]
- Slovenian Research Agency [P1-0245]
- Italian Ministry of Foreign Affairs and International Cooperation (MAECI) [PGR02952]
- [BI-RS/16-17-039]
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
- Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43009)
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
- Application of functionalyzed carbon nanotubes and gold nanoparticles for preparation of dendritic cells for tumor therapy (RS-MESTD-Basic Research (BR or ON)-175102)
DOI: 10.1016/j.msec.2018.11.073
ISSN: 0928-4931
PubMed: 30606591
WoS: 000456760700080
Scopus: 2-s2.0-85057758564
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Filipović, Nenad AU - Veselinović, Ljiljana AU - Razić, Slavica AU - Jeremić, Sanja AU - Filipić, Metka AU - Zegura, Bojana AU - Tomić, Sergej AU - Čolić, Miodrag AU - Stevanović, Magdalena PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1292 AB - Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. PB - Elsevier, Amsterdam T2 - Materials Science & Engineering C-Materials For Biological Applications T1 - Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles EP - 789 SP - 776 VL - 96 DO - 10.1016/j.msec.2018.11.073 ER -
@article{ author = "Filipović, Nenad and Veselinović, Ljiljana and Razić, Slavica and Jeremić, Sanja and Filipić, Metka and Zegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena", year = "2019", abstract = "Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.", publisher = "Elsevier, Amsterdam", journal = "Materials Science & Engineering C-Materials For Biological Applications", title = "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles", pages = "789-776", volume = "96", doi = "10.1016/j.msec.2018.11.073" }
Filipović, N., Veselinović, L., Razić, S., Jeremić, S., Filipić, M., Zegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications Elsevier, Amsterdam., 96, 776-789. https://doi.org/10.1016/j.msec.2018.11.073
Filipović N, Veselinović L, Razić S, Jeremić S, Filipić M, Zegura B, Tomić S, Čolić M, Stevanović M. Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications. 2019;96:776-789. doi:10.1016/j.msec.2018.11.073 .
Filipović, Nenad, Veselinović, Ljiljana, Razić, Slavica, Jeremić, Sanja, Filipić, Metka, Zegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science & Engineering C-Materials For Biological Applications, 96 (2019):776-789, https://doi.org/10.1016/j.msec.2018.11.073 . .