Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
Аутори
Vidić, JasminaVizzini, Priya
Manzano, Marisa
Kavanaugh, Devon
Ramarao, Nalini
Živković, Milica
Radonić, Vasa
Knezević, Nikola
Giouroudi, Ioanna
Gadjanski, Ivana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.
Кључне речи:
RNA extraction / point-of-need DNA detection / microfluidic / food security / DNA / DNA amplification / biosensorИзвор:
Sensors, 2019, 19, 5Издавач:
- MDPI, Basel
Финансирање / пројекти:
- Institut National de la Recherche Agronomique and Biosense
- ANTARES project from the European Union's Horizon 2020 research and innovation programme [664387]
- bilateral Franco-Italian Galilee Grant [37184VG]
- TOX-detect One Health EJP Project of the European Union's Horizon 2020 research and innovation programme [773830]
DOI: 10.3390/s19051100
ISSN: 1424-8220
PubMed: 30836707
WoS: 000462540400119
Scopus: 2-s2.0-85062596975
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Vidić, Jasmina AU - Vizzini, Priya AU - Manzano, Marisa AU - Kavanaugh, Devon AU - Ramarao, Nalini AU - Živković, Milica AU - Radonić, Vasa AU - Knezević, Nikola AU - Giouroudi, Ioanna AU - Gadjanski, Ivana PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1296 AB - Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification. PB - MDPI, Basel T2 - Sensors T1 - Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria IS - 5 VL - 19 DO - 10.3390/s19051100 ER -
@article{ author = "Vidić, Jasmina and Vizzini, Priya and Manzano, Marisa and Kavanaugh, Devon and Ramarao, Nalini and Živković, Milica and Radonić, Vasa and Knezević, Nikola and Giouroudi, Ioanna and Gadjanski, Ivana", year = "2019", abstract = "Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.", publisher = "MDPI, Basel", journal = "Sensors", title = "Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria", number = "5", volume = "19", doi = "10.3390/s19051100" }
Vidić, J., Vizzini, P., Manzano, M., Kavanaugh, D., Ramarao, N., Živković, M., Radonić, V., Knezević, N., Giouroudi, I.,& Gadjanski, I.. (2019). Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria. in Sensors MDPI, Basel., 19(5). https://doi.org/10.3390/s19051100
Vidić J, Vizzini P, Manzano M, Kavanaugh D, Ramarao N, Živković M, Radonić V, Knezević N, Giouroudi I, Gadjanski I. Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria. in Sensors. 2019;19(5). doi:10.3390/s19051100 .
Vidić, Jasmina, Vizzini, Priya, Manzano, Marisa, Kavanaugh, Devon, Ramarao, Nalini, Živković, Milica, Radonić, Vasa, Knezević, Nikola, Giouroudi, Ioanna, Gadjanski, Ivana, "Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria" in Sensors, 19, no. 5 (2019), https://doi.org/10.3390/s19051100 . .