Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models
Autori
Zong, YananPruner, Iva
Antović, Aleksandra
Taxiarchis, Apostolos
Vila, Zara Pons
Soutari, Nida
Mobarrez, Fariborz
Chaireti, Roza
Widengren, Jerker
Piguet, Joachim
Antović, Jovan P.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Circulating microparticles (MPs) are procoagulant due to the surface containing phosphatidylserine (PS), which facilitates coagulation. We investigated if MPs improve hemostasis in HA plasma models. MPs isolated from pooled normal human plasma were added to severe, moderate and mild HA plasma models (0%, 2.5%, 20% FVIII). The MPs' effect on hemostasis was evaluated by calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP) assays, while fibrin structure was imaged by standard confocal, stimulated emission depletion (STED) microscopy and scanning electron microscopy (SEM). MPs partially restored thrombin generation and fibrin formation in all HA plasma models. The procoagulant effect of MPs requires PS exposure, to a less extent of contact pathway activation, but not tissue factor exposure or in vitro stimulation of MPs. MPs partially normalized the fibrin structure, and using super-resolution STED, MPs attached to fibrin were clearly resolved. In summary, our resu...lts demonstrate that PS positive MPs could improve hemostasis in HA plasma models.
Izvor:
Scientific Reports, 2020, 10, 1Izdavač:
- Nature Portfolio, Berlin
Finansiranje / projekti:
- Baxalta US Inc, a member of Takeda group of companies [IIR-001459]
- China Scholarship Council (CSC)
- Karolinska Institute
DOI: 10.1038/s41598-020-64686-x
ISSN: 2045-2322
PubMed: 32398812
WoS: 000559780100041
Scopus: 2-s2.0-85084482870
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Zong, Yanan AU - Pruner, Iva AU - Antović, Aleksandra AU - Taxiarchis, Apostolos AU - Vila, Zara Pons AU - Soutari, Nida AU - Mobarrez, Fariborz AU - Chaireti, Roza AU - Widengren, Jerker AU - Piguet, Joachim AU - Antović, Jovan P. PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1302 AB - Circulating microparticles (MPs) are procoagulant due to the surface containing phosphatidylserine (PS), which facilitates coagulation. We investigated if MPs improve hemostasis in HA plasma models. MPs isolated from pooled normal human plasma were added to severe, moderate and mild HA plasma models (0%, 2.5%, 20% FVIII). The MPs' effect on hemostasis was evaluated by calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP) assays, while fibrin structure was imaged by standard confocal, stimulated emission depletion (STED) microscopy and scanning electron microscopy (SEM). MPs partially restored thrombin generation and fibrin formation in all HA plasma models. The procoagulant effect of MPs requires PS exposure, to a less extent of contact pathway activation, but not tissue factor exposure or in vitro stimulation of MPs. MPs partially normalized the fibrin structure, and using super-resolution STED, MPs attached to fibrin were clearly resolved. In summary, our results demonstrate that PS positive MPs could improve hemostasis in HA plasma models. PB - Nature Portfolio, Berlin T2 - Scientific Reports T1 - Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models IS - 1 VL - 10 DO - 10.1038/s41598-020-64686-x ER -
@article{ author = "Zong, Yanan and Pruner, Iva and Antović, Aleksandra and Taxiarchis, Apostolos and Vila, Zara Pons and Soutari, Nida and Mobarrez, Fariborz and Chaireti, Roza and Widengren, Jerker and Piguet, Joachim and Antović, Jovan P.", year = "2020", abstract = "Circulating microparticles (MPs) are procoagulant due to the surface containing phosphatidylserine (PS), which facilitates coagulation. We investigated if MPs improve hemostasis in HA plasma models. MPs isolated from pooled normal human plasma were added to severe, moderate and mild HA plasma models (0%, 2.5%, 20% FVIII). The MPs' effect on hemostasis was evaluated by calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP) assays, while fibrin structure was imaged by standard confocal, stimulated emission depletion (STED) microscopy and scanning electron microscopy (SEM). MPs partially restored thrombin generation and fibrin formation in all HA plasma models. The procoagulant effect of MPs requires PS exposure, to a less extent of contact pathway activation, but not tissue factor exposure or in vitro stimulation of MPs. MPs partially normalized the fibrin structure, and using super-resolution STED, MPs attached to fibrin were clearly resolved. In summary, our results demonstrate that PS positive MPs could improve hemostasis in HA plasma models.", publisher = "Nature Portfolio, Berlin", journal = "Scientific Reports", title = "Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models", number = "1", volume = "10", doi = "10.1038/s41598-020-64686-x" }
Zong, Y., Pruner, I., Antović, A., Taxiarchis, A., Vila, Z. P., Soutari, N., Mobarrez, F., Chaireti, R., Widengren, J., Piguet, J.,& Antović, J. P.. (2020). Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models. in Scientific Reports Nature Portfolio, Berlin., 10(1). https://doi.org/10.1038/s41598-020-64686-x
Zong Y, Pruner I, Antović A, Taxiarchis A, Vila ZP, Soutari N, Mobarrez F, Chaireti R, Widengren J, Piguet J, Antović JP. Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models. in Scientific Reports. 2020;10(1). doi:10.1038/s41598-020-64686-x .
Zong, Yanan, Pruner, Iva, Antović, Aleksandra, Taxiarchis, Apostolos, Vila, Zara Pons, Soutari, Nida, Mobarrez, Fariborz, Chaireti, Roza, Widengren, Jerker, Piguet, Joachim, Antović, Jovan P., "Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models" in Scientific Reports, 10, no. 1 (2020), https://doi.org/10.1038/s41598-020-64686-x . .