Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract
Нема приказа
Аутори
Stojković, DejanDrakulić, Danijela
Gašić, Uroš
Zengin, Gokhan
Stevanović, Milena
Rajcević, Nemanja
Soković, Marina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study explored the chemical profile of the aerial parts ofOnonis spinosaand further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential againstStaphylococcus aureusand was able to supress the production of staphyloxanthin inS. aureusat sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human can...cer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.
Извор:
Food & Function, 2020, 11, 8, 7138-7151Издавач:
- Royal Soc Chemistry, Cambridge
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
DOI: 10.1039/d0fo01595d
ISSN: 2042-6496
PubMed: 32749435
WoS: 000560678100030
Scopus: 2-s2.0-85089768738
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stojković, Dejan AU - Drakulić, Danijela AU - Gašić, Uroš AU - Zengin, Gokhan AU - Stevanović, Milena AU - Rajcević, Nemanja AU - Soković, Marina PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1312 AB - This study explored the chemical profile of the aerial parts ofOnonis spinosaand further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential againstStaphylococcus aureusand was able to supress the production of staphyloxanthin inS. aureusat sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line. PB - Royal Soc Chemistry, Cambridge T2 - Food & Function T1 - Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract EP - 7151 IS - 8 SP - 7138 VL - 11 DO - 10.1039/d0fo01595d ER -
@article{ author = "Stojković, Dejan and Drakulić, Danijela and Gašić, Uroš and Zengin, Gokhan and Stevanović, Milena and Rajcević, Nemanja and Soković, Marina", year = "2020", abstract = "This study explored the chemical profile of the aerial parts ofOnonis spinosaand further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential againstStaphylococcus aureusand was able to supress the production of staphyloxanthin inS. aureusat sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.", publisher = "Royal Soc Chemistry, Cambridge", journal = "Food & Function", title = "Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract", pages = "7151-7138", number = "8", volume = "11", doi = "10.1039/d0fo01595d" }
Stojković, D., Drakulić, D., Gašić, U., Zengin, G., Stevanović, M., Rajcević, N.,& Soković, M.. (2020). Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function Royal Soc Chemistry, Cambridge., 11(8), 7138-7151. https://doi.org/10.1039/d0fo01595d
Stojković D, Drakulić D, Gašić U, Zengin G, Stevanović M, Rajcević N, Soković M. Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function. 2020;11(8):7138-7151. doi:10.1039/d0fo01595d .
Stojković, Dejan, Drakulić, Danijela, Gašić, Uroš, Zengin, Gokhan, Stevanović, Milena, Rajcević, Nemanja, Soković, Marina, "Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract" in Food & Function, 11, no. 8 (2020):7138-7151, https://doi.org/10.1039/d0fo01595d . .