GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro
Аутори
Soković Bajić, SvetlanaĐokić, Jelena
Dinić, Miroslav
Tomić, Sergej
Popović, Nikola
Brdarić, Emilija
Golić, Nataša
Tolinački, Maja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By... looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.
Извор:
Scientific Reports, 2020, 10, 1347-Издавач:
- Nature Publishing Group, London
Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
- Молекуларна карактеризација тумора штитасте жлезде: биолошки и клинички аспекти (RS-MESTD-Basic Research (BR or ON)-173050)
- Примена функционализованих угљеничних наноцеви и наночестица злата за припрему дендритских ћелија у терапији тумора (RS-MESTD-Basic Research (BR or ON)-175102)
DOI: 10.1038/s41598-020-58177-2
ISSN: 2045-2322
PubMed: 31992761
WoS: 000562863800001
Scopus: 2-s2.0-85078434783
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Soković Bajić, Svetlana AU - Đokić, Jelena AU - Dinić, Miroslav AU - Tomić, Sergej AU - Popović, Nikola AU - Brdarić, Emilija AU - Golić, Nataša AU - Tolinački, Maja PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1318 AB - The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects. PB - Nature Publishing Group, London T2 - Scientific Reports T1 - GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro SP - 1347 VL - 10 DO - 10.1038/s41598-020-58177-2 ER -
@article{ author = "Soković Bajić, Svetlana and Đokić, Jelena and Dinić, Miroslav and Tomić, Sergej and Popović, Nikola and Brdarić, Emilija and Golić, Nataša and Tolinački, Maja", year = "2020", abstract = "The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.", publisher = "Nature Publishing Group, London", journal = "Scientific Reports", title = "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro", pages = "1347", volume = "10", doi = "10.1038/s41598-020-58177-2" }
Soković Bajić, S., Đokić, J., Dinić, M., Tomić, S., Popović, N., Brdarić, E., Golić, N.,& Tolinački, M.. (2020). GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports Nature Publishing Group, London., 10, 1347. https://doi.org/10.1038/s41598-020-58177-2
Soković Bajić S, Đokić J, Dinić M, Tomić S, Popović N, Brdarić E, Golić N, Tolinački M. GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports. 2020;10:1347. doi:10.1038/s41598-020-58177-2 .
Soković Bajić, Svetlana, Đokić, Jelena, Dinić, Miroslav, Tomić, Sergej, Popović, Nikola, Brdarić, Emilija, Golić, Nataša, Tolinački, Maja, "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro" in Scientific Reports, 10 (2020):1347, https://doi.org/10.1038/s41598-020-58177-2 . .