Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts
Authors
Jovanović, MarinaMorić, Ivana
Nikolić, Biljana
Pavić, Aleksandar
Svircev, Emilija
Šenerović, Lidija
Mitić-Culafić, Dragana
Article (Published version)
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Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentr...ations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.
Keywords:
zebrafish / Persicaria maculosa / Bistorta officinalis / anti-quorum sensing activity / antibiofilm activitySource:
Molecules, 2020, 25, 8Publisher:
- MDPI, Basel
Funding / projects:
- Institute of General and Physical Chemistry, Serbia
- Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-MESTD-Basic Research (BR or ON)-172058)
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.3390/molecules25081811
ISSN: 1420-3049
PubMed: 32326481
WoS: 000534617300031
Scopus: 2-s2.0-85083675642
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovanović, Marina AU - Morić, Ivana AU - Nikolić, Biljana AU - Pavić, Aleksandar AU - Svircev, Emilija AU - Šenerović, Lidija AU - Mitić-Culafić, Dragana PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1320 AB - Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections. PB - MDPI, Basel T2 - Molecules T1 - Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts IS - 8 VL - 25 DO - 10.3390/molecules25081811 ER -
@article{ author = "Jovanović, Marina and Morić, Ivana and Nikolić, Biljana and Pavić, Aleksandar and Svircev, Emilija and Šenerović, Lidija and Mitić-Culafić, Dragana", year = "2020", abstract = "Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.", publisher = "MDPI, Basel", journal = "Molecules", title = "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts", number = "8", volume = "25", doi = "10.3390/molecules25081811" }
Jovanović, M., Morić, I., Nikolić, B., Pavić, A., Svircev, E., Šenerović, L.,& Mitić-Culafić, D.. (2020). Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules MDPI, Basel., 25(8). https://doi.org/10.3390/molecules25081811
Jovanović M, Morić I, Nikolić B, Pavić A, Svircev E, Šenerović L, Mitić-Culafić D. Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules. 2020;25(8). doi:10.3390/molecules25081811 .
Jovanović, Marina, Morić, Ivana, Nikolić, Biljana, Pavić, Aleksandar, Svircev, Emilija, Šenerović, Lidija, Mitić-Culafić, Dragana, "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts" in Molecules, 25, no. 8 (2020), https://doi.org/10.3390/molecules25081811 . .