Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging
Нема приказа
Аутори
Kurutos, AtanasIlić-Tomić, Tatjana
Kamounah, Fadhil S.
Vasilev, Aleksey A.
Nikodinović-Runić, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cyanine based chemosensing platforms have successfully been employed over the past couple of decades in various fields of biomolecular sciences. Still a substantial number of recent advances and improvements on this class of compounds are reported in the art. This paper presents our latest work on the improved synthetic approach, study on photophysical properties, and biosensing applicability of monomethine cyanine dyes. The series of mono-, di- and tricationic dyes showed up to 5-fold enhanced resistance against photobleaching compared to the commercially available Thiazole Orange (TO). The title compounds were studied as potential molecular probes for the detection of deoxyribonucleic acid, demonstrating their capacity as excellent fluorescent labeling agents. Depending on the dye chemical structure, current Cl-TO compounds exhibit up to 834-fold enhanced fluorescence emission and form stable complexes with Calf Thymus-DNA. The calculated binding constants were found to be higher tha...n several conventional fluorogenic dyes for nucleic acid detection. All studied derivatives appeared as less cytotoxic than the Thiazole Orange. IC50 concentrations in human fibro-blasts MRCS cell line were calculated up to 50 mu M for the synthesized Cl-TO dyes, and 0.5 mu M for the parental Thiazole Orange. Two of the dyes were found very competent in post-electrophoretic visualization of DNA. As demonstrated by the agarose gel electrophoresis, the staining efficiency and detection limits of the dyes were comparable to the widely used Ethidium Bromide. The tricationic dye revealed great potential for cell cycle analysis in G1, S and G2 phases. The chlorinated TO derivatives readily stain human cells in vivo, while they can effectively be applied for eukaryotic and microbial cell staining.
Кључне речи:
Nucleic acid detection / Non-Cytotoxic molecular probes / Microbial cell staining / Fluorescent microscopy / Cyanine dyesИзвор:
Journal of Photochemistry and Photobiology A-Chemistry, 2020, 397Издавач:
- Elsevier Science Sa, Lausanne
Финансирање / пројекти:
- Bulgarian National Science Fund (BNSF) [DM09/5]
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
- Bulgarian National Science Fund (BNSF) project SOFIa [KP-06-H39/11]
DOI: 10.1016/j.jphotochem.2020.112598
ISSN: 1010-6030
WoS: 000539349900018
Scopus: 2-s2.0-85084353961
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kurutos, Atanas AU - Ilić-Tomić, Tatjana AU - Kamounah, Fadhil S. AU - Vasilev, Aleksey A. AU - Nikodinović-Runić, Jasmina PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1328 AB - Cyanine based chemosensing platforms have successfully been employed over the past couple of decades in various fields of biomolecular sciences. Still a substantial number of recent advances and improvements on this class of compounds are reported in the art. This paper presents our latest work on the improved synthetic approach, study on photophysical properties, and biosensing applicability of monomethine cyanine dyes. The series of mono-, di- and tricationic dyes showed up to 5-fold enhanced resistance against photobleaching compared to the commercially available Thiazole Orange (TO). The title compounds were studied as potential molecular probes for the detection of deoxyribonucleic acid, demonstrating their capacity as excellent fluorescent labeling agents. Depending on the dye chemical structure, current Cl-TO compounds exhibit up to 834-fold enhanced fluorescence emission and form stable complexes with Calf Thymus-DNA. The calculated binding constants were found to be higher than several conventional fluorogenic dyes for nucleic acid detection. All studied derivatives appeared as less cytotoxic than the Thiazole Orange. IC50 concentrations in human fibro-blasts MRCS cell line were calculated up to 50 mu M for the synthesized Cl-TO dyes, and 0.5 mu M for the parental Thiazole Orange. Two of the dyes were found very competent in post-electrophoretic visualization of DNA. As demonstrated by the agarose gel electrophoresis, the staining efficiency and detection limits of the dyes were comparable to the widely used Ethidium Bromide. The tricationic dye revealed great potential for cell cycle analysis in G1, S and G2 phases. The chlorinated TO derivatives readily stain human cells in vivo, while they can effectively be applied for eukaryotic and microbial cell staining. PB - Elsevier Science Sa, Lausanne T2 - Journal of Photochemistry and Photobiology A-Chemistry T1 - Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging VL - 397 DO - 10.1016/j.jphotochem.2020.112598 ER -
@article{ author = "Kurutos, Atanas and Ilić-Tomić, Tatjana and Kamounah, Fadhil S. and Vasilev, Aleksey A. and Nikodinović-Runić, Jasmina", year = "2020", abstract = "Cyanine based chemosensing platforms have successfully been employed over the past couple of decades in various fields of biomolecular sciences. Still a substantial number of recent advances and improvements on this class of compounds are reported in the art. This paper presents our latest work on the improved synthetic approach, study on photophysical properties, and biosensing applicability of monomethine cyanine dyes. The series of mono-, di- and tricationic dyes showed up to 5-fold enhanced resistance against photobleaching compared to the commercially available Thiazole Orange (TO). The title compounds were studied as potential molecular probes for the detection of deoxyribonucleic acid, demonstrating their capacity as excellent fluorescent labeling agents. Depending on the dye chemical structure, current Cl-TO compounds exhibit up to 834-fold enhanced fluorescence emission and form stable complexes with Calf Thymus-DNA. The calculated binding constants were found to be higher than several conventional fluorogenic dyes for nucleic acid detection. All studied derivatives appeared as less cytotoxic than the Thiazole Orange. IC50 concentrations in human fibro-blasts MRCS cell line were calculated up to 50 mu M for the synthesized Cl-TO dyes, and 0.5 mu M for the parental Thiazole Orange. Two of the dyes were found very competent in post-electrophoretic visualization of DNA. As demonstrated by the agarose gel electrophoresis, the staining efficiency and detection limits of the dyes were comparable to the widely used Ethidium Bromide. The tricationic dye revealed great potential for cell cycle analysis in G1, S and G2 phases. The chlorinated TO derivatives readily stain human cells in vivo, while they can effectively be applied for eukaryotic and microbial cell staining.", publisher = "Elsevier Science Sa, Lausanne", journal = "Journal of Photochemistry and Photobiology A-Chemistry", title = "Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging", volume = "397", doi = "10.1016/j.jphotochem.2020.112598" }
Kurutos, A., Ilić-Tomić, T., Kamounah, F. S., Vasilev, A. A.,& Nikodinović-Runić, J.. (2020). Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging. in Journal of Photochemistry and Photobiology A-Chemistry Elsevier Science Sa, Lausanne., 397. https://doi.org/10.1016/j.jphotochem.2020.112598
Kurutos A, Ilić-Tomić T, Kamounah FS, Vasilev AA, Nikodinović-Runić J. Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging. in Journal of Photochemistry and Photobiology A-Chemistry. 2020;397. doi:10.1016/j.jphotochem.2020.112598 .
Kurutos, Atanas, Ilić-Tomić, Tatjana, Kamounah, Fadhil S., Vasilev, Aleksey A., Nikodinović-Runić, Jasmina, "Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging" in Journal of Photochemistry and Photobiology A-Chemistry, 397 (2020), https://doi.org/10.1016/j.jphotochem.2020.112598 . .