Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci
2020
Autori
Kojić, MilanJovčić, Branko
Miljković, Marija
Novović, Katarina
Begović, Jelena
Studholme, David J.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Bacteria can gain resistance to antimicrobials by acquiring and expressing genetic elements that encode resistance determinants such as efflux pumps and drug-modifying enzymes, thus hampering treatment of infection. Previously we showed that acquisition of spectinomycin resistance in a lactococcal strain was correlated with a reversible genomic inversion, but the precise location and the genes affected were unknown. Here we use long read whole-genome sequencing to precisely define the genomic inversion and we use quantitative PCR to identify associated changes in gene expression levels. The boundaries of the inversion fall within two identical copies of a prophage-like sequence, located on the left and right replichores; this suggests possible mechanisms for inversion through homologous recombination or prophage activity. The inversion is asymmetrical in respect of the axis between the origin and terminus of the replication and modulates the expression of a SAM-dependent methyltransfer...ase, whose heterologous expression confers resistance to spectinomycin in lactococci and that is up regulated on exposure to spectinomycin. This study provides one of the first examples of phase variation via large-scale chromosomal inversions that confers a switch in antimicrobial resistance in bacteria and the first outside of Staphylococcus aureus.
Ključne reči:
Spectinomycin resistance / SAM-dependent methyltransferase / Reversion / Prophages / Phase variation / Chromosomal flip-flop reversible inversionIzvor:
Microbiological Research, 2020, 241Izdavač:
- Elsevier Gmbh, Munich
Finansiranje / projekti:
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
- ICGEB Collaborative research grants [CRP/YUG10-01, CRP/SRB15-02]
DOI: 10.1016/j.micres.2020.126583
ISSN: 0944-5013
PubMed: 32919223
WoS: 000587293300010
Scopus: 2-s2.0-85090406249
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milan AU - Jovčić, Branko AU - Miljković, Marija AU - Novović, Katarina AU - Begović, Jelena AU - Studholme, David J. PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1373 AB - Bacteria can gain resistance to antimicrobials by acquiring and expressing genetic elements that encode resistance determinants such as efflux pumps and drug-modifying enzymes, thus hampering treatment of infection. Previously we showed that acquisition of spectinomycin resistance in a lactococcal strain was correlated with a reversible genomic inversion, but the precise location and the genes affected were unknown. Here we use long read whole-genome sequencing to precisely define the genomic inversion and we use quantitative PCR to identify associated changes in gene expression levels. The boundaries of the inversion fall within two identical copies of a prophage-like sequence, located on the left and right replichores; this suggests possible mechanisms for inversion through homologous recombination or prophage activity. The inversion is asymmetrical in respect of the axis between the origin and terminus of the replication and modulates the expression of a SAM-dependent methyltransferase, whose heterologous expression confers resistance to spectinomycin in lactococci and that is up regulated on exposure to spectinomycin. This study provides one of the first examples of phase variation via large-scale chromosomal inversions that confers a switch in antimicrobial resistance in bacteria and the first outside of Staphylococcus aureus. PB - Elsevier Gmbh, Munich T2 - Microbiological Research T1 - Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci VL - 241 DO - 10.1016/j.micres.2020.126583 ER -
@article{ author = "Kojić, Milan and Jovčić, Branko and Miljković, Marija and Novović, Katarina and Begović, Jelena and Studholme, David J.", year = "2020", abstract = "Bacteria can gain resistance to antimicrobials by acquiring and expressing genetic elements that encode resistance determinants such as efflux pumps and drug-modifying enzymes, thus hampering treatment of infection. Previously we showed that acquisition of spectinomycin resistance in a lactococcal strain was correlated with a reversible genomic inversion, but the precise location and the genes affected were unknown. Here we use long read whole-genome sequencing to precisely define the genomic inversion and we use quantitative PCR to identify associated changes in gene expression levels. The boundaries of the inversion fall within two identical copies of a prophage-like sequence, located on the left and right replichores; this suggests possible mechanisms for inversion through homologous recombination or prophage activity. The inversion is asymmetrical in respect of the axis between the origin and terminus of the replication and modulates the expression of a SAM-dependent methyltransferase, whose heterologous expression confers resistance to spectinomycin in lactococci and that is up regulated on exposure to spectinomycin. This study provides one of the first examples of phase variation via large-scale chromosomal inversions that confers a switch in antimicrobial resistance in bacteria and the first outside of Staphylococcus aureus.", publisher = "Elsevier Gmbh, Munich", journal = "Microbiological Research", title = "Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci", volume = "241", doi = "10.1016/j.micres.2020.126583" }
Kojić, M., Jovčić, B., Miljković, M., Novović, K., Begović, J.,& Studholme, D. J.. (2020). Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci. in Microbiological Research Elsevier Gmbh, Munich., 241. https://doi.org/10.1016/j.micres.2020.126583
Kojić M, Jovčić B, Miljković M, Novović K, Begović J, Studholme DJ. Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci. in Microbiological Research. 2020;241. doi:10.1016/j.micres.2020.126583 .
Kojić, Milan, Jovčić, Branko, Miljković, Marija, Novović, Katarina, Begović, Jelena, Studholme, David J., "Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci" in Microbiological Research, 241 (2020), https://doi.org/10.1016/j.micres.2020.126583 . .