Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
2020
Authors
Mojicević, MarijaD'Agostino, Paul M.
Pavić, Aleksandar
Vojnović, Sandra
Senthamaraikannan, Ramsankar
Vasiljević, Branka
Gulder, Tobias A. M.
Nikodinović-Runić, Jasmina
Article (Published version)
Metadata
Show full item recordAbstract
Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endotheli...al cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.
Keywords:
zebrafish / Streptomyces sp. / staurosporine / secondary metabolite / antifungal activity / antiangiogenesisSource:
Microbiologyopen, 2020, 9, 3Publisher:
- Wiley, Hoboken
Funding / projects:
- Horizon 2020 Framework Programme [745435]
- Deutsche Forschungsgemeinschaft [GU 1233/1-1]
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
- Deutscher Akademischer Austauschdienst
DOI: 10.1002/mbo3.986
ISSN: 2045-8827
PubMed: 31989798
WoS: 000521113200011
Scopus: 2-s2.0-85078776914
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mojicević, Marija AU - D'Agostino, Paul M. AU - Pavić, Aleksandar AU - Vojnović, Sandra AU - Senthamaraikannan, Ramsankar AU - Vasiljević, Branka AU - Gulder, Tobias A. M. AU - Nikodinović-Runić, Jasmina PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1384 AB - Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability. PB - Wiley, Hoboken T2 - Microbiologyopen T1 - Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties IS - 3 VL - 9 DO - 10.1002/mbo3.986 ER -
@article{ author = "Mojicević, Marija and D'Agostino, Paul M. and Pavić, Aleksandar and Vojnović, Sandra and Senthamaraikannan, Ramsankar and Vasiljević, Branka and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina", year = "2020", abstract = "Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.", publisher = "Wiley, Hoboken", journal = "Microbiologyopen", title = "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties", number = "3", volume = "9", doi = "10.1002/mbo3.986" }
Mojicević, M., D'Agostino, P. M., Pavić, A., Vojnović, S., Senthamaraikannan, R., Vasiljević, B., Gulder, T. A. M.,& Nikodinović-Runić, J.. (2020). Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen Wiley, Hoboken., 9(3). https://doi.org/10.1002/mbo3.986
Mojicević M, D'Agostino PM, Pavić A, Vojnović S, Senthamaraikannan R, Vasiljević B, Gulder TAM, Nikodinović-Runić J. Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen. 2020;9(3). doi:10.1002/mbo3.986 .
Mojicević, Marija, D'Agostino, Paul M., Pavić, Aleksandar, Vojnović, Sandra, Senthamaraikannan, Ramsankar, Vasiljević, Branka, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties" in Microbiologyopen, 9, no. 3 (2020), https://doi.org/10.1002/mbo3.986 . .