The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11
Нема приказа
Аутори
Živković, MilicaSoković Bajić, Svetlana
Tolinački, Maja
Brdarić, Emilija
Đokić, Jelena
Popović, Nikola
Rajić, Jovana
Đorđević, Marija
Golić, Nataša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results... The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.
Извор:
Journal of Clinical Gastroenterology, 2020, 54, S11-S12Издавач:
- Wolters Kluwer Health
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
Напомена:
- 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. Wolters Kluwer Health; 2020. p. 103. (Journal of Clinical Gastroenterology; Vol. 54; Suppl. 1)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Živković, Milica AU - Soković Bajić, Svetlana AU - Tolinački, Maja AU - Brdarić, Emilija AU - Đokić, Jelena AU - Popović, Nikola AU - Rajić, Jovana AU - Đorđević, Marija AU - Golić, Nataša PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1395 AB - Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota. PB - Wolters Kluwer Health C3 - Journal of Clinical Gastroenterology T1 - The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11 EP - S12 SP - S11 VL - 54 DO - 10.1097/MCG.0000000000001292 UR - https://hdl.handle.net/21.15107/rcub_imagine_1395 ER -
@conference{ author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša", year = "2020", abstract = "Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.", publisher = "Wolters Kluwer Health", journal = "Journal of Clinical Gastroenterology", title = "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11", pages = "S12-S11", volume = "54", doi = "10.1097/MCG.0000000000001292", url = "https://hdl.handle.net/21.15107/rcub_imagine_1395" }
Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2020). The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology Wolters Kluwer Health., 54, S11-S12. https://doi.org/10.1097/MCG.0000000000001292 https://hdl.handle.net/21.15107/rcub_imagine_1395
Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology. 2020;54:S11-S12. doi:10.1097/MCG.0000000000001292 https://hdl.handle.net/21.15107/rcub_imagine_1395 .
Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11" in Journal of Clinical Gastroenterology, 54 (2020):S11-S12, https://doi.org/10.1097/MCG.0000000000001292 ., https://hdl.handle.net/21.15107/rcub_imagine_1395 .