Facile Synthesis of L-Cysteine Functionalized Graphene Quantum Dots as a Bioimaging and Photosensitive Agent
Autori
Milenković, MilaMisović, Aleksandra
Jovanović, Dragana
Popović Bijelić, Ana
Ciasca, Gabriele
Romano, Sabrina
Bonasera, Aurelio
Mojsin, Marija
Pejić, Jelena
Stevanović, Milena
Jovanović, Svetlana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in al...l gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs.
Ključne reči:
photoluminescence / photodynamic therapy / graphene quantum dots / gamma irradiation / bioimaging / atomic force microscopyIzvor:
Nanomaterials, 2021, 11, 8, 1879-Izdavač:
- MDPI, Basel
Finansiranje / projekti:
- Biomarkeri u neurodegenerativnim i malignim procesima (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
- Italian Ministry of University and Research (MURST, ex-MIUR) [CUP B78D19000280001]
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200017 (Univerzitet u Beogradu, Institut za nuklearne nauke Vinča, Beograd-Vinča) (RS-MESTD-inst-2020-200017)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
DOI: 10.3390/nano11081879
ISSN: 2079-4991
WoS: 000690115800001
Scopus: 2-s2.0-85110612605
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Milenković, Mila AU - Misović, Aleksandra AU - Jovanović, Dragana AU - Popović Bijelić, Ana AU - Ciasca, Gabriele AU - Romano, Sabrina AU - Bonasera, Aurelio AU - Mojsin, Marija AU - Pejić, Jelena AU - Stevanović, Milena AU - Jovanović, Svetlana PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1457 AB - Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in all gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs. PB - MDPI, Basel T2 - Nanomaterials T1 - Facile Synthesis of L-Cysteine Functionalized Graphene Quantum Dots as a Bioimaging and Photosensitive Agent IS - 8 SP - 1879 VL - 11 DO - 10.3390/nano11081879 ER -
@article{ author = "Milenković, Mila and Misović, Aleksandra and Jovanović, Dragana and Popović Bijelić, Ana and Ciasca, Gabriele and Romano, Sabrina and Bonasera, Aurelio and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena and Jovanović, Svetlana", year = "2021", abstract = "Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in all gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs.", publisher = "MDPI, Basel", journal = "Nanomaterials", title = "Facile Synthesis of L-Cysteine Functionalized Graphene Quantum Dots as a Bioimaging and Photosensitive Agent", number = "8", pages = "1879", volume = "11", doi = "10.3390/nano11081879" }
Milenković, M., Misović, A., Jovanović, D., Popović Bijelić, A., Ciasca, G., Romano, S., Bonasera, A., Mojsin, M., Pejić, J., Stevanović, M.,& Jovanović, S.. (2021). Facile Synthesis of L-Cysteine Functionalized Graphene Quantum Dots as a Bioimaging and Photosensitive Agent. in Nanomaterials MDPI, Basel., 11(8), 1879. https://doi.org/10.3390/nano11081879
Milenković M, Misović A, Jovanović D, Popović Bijelić A, Ciasca G, Romano S, Bonasera A, Mojsin M, Pejić J, Stevanović M, Jovanović S. Facile Synthesis of L-Cysteine Functionalized Graphene Quantum Dots as a Bioimaging and Photosensitive Agent. in Nanomaterials. 2021;11(8):1879. doi:10.3390/nano11081879 .
Milenković, Mila, Misović, Aleksandra, Jovanović, Dragana, Popović Bijelić, Ana, Ciasca, Gabriele, Romano, Sabrina, Bonasera, Aurelio, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena, Jovanović, Svetlana, "Facile Synthesis of L-Cysteine Functionalized Graphene Quantum Dots as a Bioimaging and Photosensitive Agent" in Nanomaterials, 11, no. 8 (2021):1879, https://doi.org/10.3390/nano11081879 . .