Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing
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Olaifa, KayodeNikodinović-Runić, Jasmina
Glišić, Biljana
Boschetto, Francesco
Marin, Elia
Segreto, Francesco
Marsili, Enrico
Article (Published version)
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Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of... electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.
Keywords:
Fluconazole / Electroanalysis / Complex Ag3 / Candida albicans / Biofilm / Amphotericin BSource:
Electrochimica Acta, 2021, 389Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Nazarbayev University, School of Engineering and Digital Sciences, Kazakhstan under the Faculty Development Competitive Research Grant Program [110119FD4537]
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200122 (University of Kragujevac, Faculty of Science) (RS-MESTD-inst-2020-200122)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
DOI: 10.1016/j.electacta.2021.138757
ISSN: 0013-4686
WoS: 000687334700006
Scopus: 2-s2.0-85109099094
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Olaifa, Kayode AU - Nikodinović-Runić, Jasmina AU - Glišić, Biljana AU - Boschetto, Francesco AU - Marin, Elia AU - Segreto, Francesco AU - Marsili, Enrico PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1465 AB - Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Electrochimica Acta T1 - Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing VL - 389 DO - 10.1016/j.electacta.2021.138757 ER -
@article{ author = "Olaifa, Kayode and Nikodinović-Runić, Jasmina and Glišić, Biljana and Boschetto, Francesco and Marin, Elia and Segreto, Francesco and Marsili, Enrico", year = "2021", abstract = "Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Electrochimica Acta", title = "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing", volume = "389", doi = "10.1016/j.electacta.2021.138757" }
Olaifa, K., Nikodinović-Runić, J., Glišić, B., Boschetto, F., Marin, E., Segreto, F.,& Marsili, E.. (2021). Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta Pergamon-Elsevier Science Ltd, Oxford., 389. https://doi.org/10.1016/j.electacta.2021.138757
Olaifa K, Nikodinović-Runić J, Glišić B, Boschetto F, Marin E, Segreto F, Marsili E. Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta. 2021;389. doi:10.1016/j.electacta.2021.138757 .
Olaifa, Kayode, Nikodinović-Runić, Jasmina, Glišić, Biljana, Boschetto, Francesco, Marin, Elia, Segreto, Francesco, Marsili, Enrico, "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing" in Electrochimica Acta, 389 (2021), https://doi.org/10.1016/j.electacta.2021.138757 . .