Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing
Nema prikaza
Autori
Olaifa, KayodeNikodinović-Runić, Jasmina
Glišić, Biljana
Boschetto, Francesco
Marin, Elia
Segreto, Francesco
Marsili, Enrico
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of... electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.
Ključne reči:
Fluconazole / Electroanalysis / Complex Ag3 / Candida albicans / Biofilm / Amphotericin BIzvor:
Electrochimica Acta, 2021, 389Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Nazarbayev University, School of Engineering and Digital Sciences, Kazakhstan under the Faculty Development Competitive Research Grant Program [110119FD4537]
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200122 (Univerzitet u Kragujevcu, Prirodno-matematički fakultet) (RS-MESTD-inst-2020-200122)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
DOI: 10.1016/j.electacta.2021.138757
ISSN: 0013-4686
WoS: 000687334700006
Scopus: 2-s2.0-85109099094
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Olaifa, Kayode AU - Nikodinović-Runić, Jasmina AU - Glišić, Biljana AU - Boschetto, Francesco AU - Marin, Elia AU - Segreto, Francesco AU - Marsili, Enrico PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1465 AB - Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Electrochimica Acta T1 - Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing VL - 389 DO - 10.1016/j.electacta.2021.138757 ER -
@article{ author = "Olaifa, Kayode and Nikodinović-Runić, Jasmina and Glišić, Biljana and Boschetto, Francesco and Marin, Elia and Segreto, Francesco and Marsili, Enrico", year = "2021", abstract = "Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Electrochimica Acta", title = "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing", volume = "389", doi = "10.1016/j.electacta.2021.138757" }
Olaifa, K., Nikodinović-Runić, J., Glišić, B., Boschetto, F., Marin, E., Segreto, F.,& Marsili, E.. (2021). Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta Pergamon-Elsevier Science Ltd, Oxford., 389. https://doi.org/10.1016/j.electacta.2021.138757
Olaifa K, Nikodinović-Runić J, Glišić B, Boschetto F, Marin E, Segreto F, Marsili E. Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta. 2021;389. doi:10.1016/j.electacta.2021.138757 .
Olaifa, Kayode, Nikodinović-Runić, Jasmina, Glišić, Biljana, Boschetto, Francesco, Marin, Elia, Segreto, Francesco, Marsili, Enrico, "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing" in Electrochimica Acta, 389 (2021), https://doi.org/10.1016/j.electacta.2021.138757 . .