Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells
2021
Autori
Brdarić, EmilijaSoković Bajić, Svetlana
Đokić, Jelena
Durdić, Sladana
Ruas-Madiedo, Patricia
Stevanović, Magdalena
Tolinački, Maja
Dinić, Miroslav
Mutić, Jelena
Golić, Nataša
Živković, Milica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.
Ključne reči:
oxidative stress / Lactiplantibacillus plantarum / intestinal epithelial cells / inflammation / exopolysaccharides / cellular junctions / cadmiumIzvor:
Frontiers in Microbiology, 2021, 12Izdavač:
- Frontiers Media Sa, Lausanne
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
DOI: 10.3389/fmicb.2021.759378
ISSN: 1664-302X
WoS: 000719997300001
Scopus: 2-s2.0-85119152907
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Brdarić, Emilija AU - Soković Bajić, Svetlana AU - Đokić, Jelena AU - Durdić, Sladana AU - Ruas-Madiedo, Patricia AU - Stevanović, Magdalena AU - Tolinački, Maja AU - Dinić, Miroslav AU - Mutić, Jelena AU - Golić, Nataša AU - Živković, Milica PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1471 AB - Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells VL - 12 DO - 10.3389/fmicb.2021.759378 ER -
@article{ author = "Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Durdić, Sladana and Ruas-Madiedo, Patricia and Stevanović, Magdalena and Tolinački, Maja and Dinić, Miroslav and Mutić, Jelena and Golić, Nataša and Živković, Milica", year = "2021", abstract = "Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells", volume = "12", doi = "10.3389/fmicb.2021.759378" }
Brdarić, E., Soković Bajić, S., Đokić, J., Durdić, S., Ruas-Madiedo, P., Stevanović, M., Tolinački, M., Dinić, M., Mutić, J., Golić, N.,& Živković, M.. (2021). Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 12. https://doi.org/10.3389/fmicb.2021.759378
Brdarić E, Soković Bajić S, Đokić J, Durdić S, Ruas-Madiedo P, Stevanović M, Tolinački M, Dinić M, Mutić J, Golić N, Živković M. Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology. 2021;12. doi:10.3389/fmicb.2021.759378 .
Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Durdić, Sladana, Ruas-Madiedo, Patricia, Stevanović, Magdalena, Tolinački, Maja, Dinić, Miroslav, Mutić, Jelena, Golić, Nataša, Živković, Milica, "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells" in Frontiers in Microbiology, 12 (2021), https://doi.org/10.3389/fmicb.2021.759378 . .