Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression
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Ušjak, DušanDinić, Miroslav
Novović, Katarina
Ivković, Branka
Filipović, Nenad
Stevanović, Magdalena
Milenković, Marina T.
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An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusi...on is that 2 '-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
Keywords:
virulence factors / polymerase chain reaction / gene expression / chalcones / Acinetobacter baumanniiSource:
Chemistry & Biodiversity, 2021, 18, 1Publisher:
- Wiley-V C H Verlag Gmbh, Weinheim
DOI: 10.1002/cbdv.202000786
ISSN: 1612-1872
PubMed: 33188577
WoS: 000596697700001
Scopus: 2-s2.0-85097239451
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ušjak, Dušan AU - Dinić, Miroslav AU - Novović, Katarina AU - Ivković, Branka AU - Filipović, Nenad AU - Stevanović, Magdalena AU - Milenković, Marina T. PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1509 AB - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2 '-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility. PB - Wiley-V C H Verlag Gmbh, Weinheim T2 - Chemistry & Biodiversity T1 - Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression IS - 1 VL - 18 DO - 10.1002/cbdv.202000786 ER -
@article{ author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.", year = "2021", abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2 '-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.", publisher = "Wiley-V C H Verlag Gmbh, Weinheim", journal = "Chemistry & Biodiversity", title = "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression", number = "1", volume = "18", doi = "10.1002/cbdv.202000786" }
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2021). Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity Wiley-V C H Verlag Gmbh, Weinheim., 18(1). https://doi.org/10.1002/cbdv.202000786
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity. 2021;18(1). doi:10.1002/cbdv.202000786 .
Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021), https://doi.org/10.1002/cbdv.202000786 . .