Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients
Authorized Users Only
2022
Authors
Tertel, TobiasTomić, Sergej
Đokić, Jelena
Radojević, Dušan
Stevanović, Dejan
Ilić, Nataša
Giebel, Bernd
Kosanović, Maja
Article (Published version)
Metadata
Show full item recordAbstract
COVID-19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role of extracellular vesicles (EVs) in COVID-19 and their biomarker potential, however, remains largely unknown. Aiming to identify specific EV signatures of patients with mild compared to severe COVID-19, we characterized the EV composition of 20 mild and 26 severe COVID-19 patients along with 16 sex and age-matched healthy donors with a panel of eight different antibodies by imaging flow cytometry (IFCM). We correlated the obtained data with 37 clinical, prerecorded biochemical and immunological parameters. Severe patients' sera contained increased amounts of CD13(+) and CD82(+) EVs, which positively correlated with IL-6-producing and circulating myeloid-derived suppressor cells (MDSCs) and with the serum concentration of proinflammatory cytokines, respectively. Sera of mild COVID-19 patients contained more HLA-ABC(+) EVs than sera of the healthy do...nors and more CD24(+) EVs than severe COVID-19 patients. Their increased abundance negatively correlated with disease severity and accumulation of MDSCs, being considered as key drivers of immunopathogenesis in COVID-19. Altogether, our results support the potential of serum EVs as powerful biomarkers for COVID-19 severity and pave the way for future investigations aiming to unravel the role of EVs in COVID-19 progression.
Keywords:
serum / MDSC / IFCM / extracellular vesicles / COVID-19 / biomarkersSource:
Journal of Extracellular Vesicles, 2022, 11, 8Publisher:
- Hoboken : Wiley
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200019 (University of Belgrade, Institute for the Application of Nuclear Energy - INEP) (RS-MESTD-inst-2020-200019)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- 451-03-921/2020-14/6
DOI: 10.1002/jev2.12257
ISSN: 2001-3078
PubMed: 35979935
WoS: 000842076100001
Scopus: 2-s2.0-85136017383
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Tertel, Tobias AU - Tomić, Sergej AU - Đokić, Jelena AU - Radojević, Dušan AU - Stevanović, Dejan AU - Ilić, Nataša AU - Giebel, Bernd AU - Kosanović, Maja PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1517 AB - COVID-19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role of extracellular vesicles (EVs) in COVID-19 and their biomarker potential, however, remains largely unknown. Aiming to identify specific EV signatures of patients with mild compared to severe COVID-19, we characterized the EV composition of 20 mild and 26 severe COVID-19 patients along with 16 sex and age-matched healthy donors with a panel of eight different antibodies by imaging flow cytometry (IFCM). We correlated the obtained data with 37 clinical, prerecorded biochemical and immunological parameters. Severe patients' sera contained increased amounts of CD13(+) and CD82(+) EVs, which positively correlated with IL-6-producing and circulating myeloid-derived suppressor cells (MDSCs) and with the serum concentration of proinflammatory cytokines, respectively. Sera of mild COVID-19 patients contained more HLA-ABC(+) EVs than sera of the healthy donors and more CD24(+) EVs than severe COVID-19 patients. Their increased abundance negatively correlated with disease severity and accumulation of MDSCs, being considered as key drivers of immunopathogenesis in COVID-19. Altogether, our results support the potential of serum EVs as powerful biomarkers for COVID-19 severity and pave the way for future investigations aiming to unravel the role of EVs in COVID-19 progression. PB - Hoboken : Wiley T2 - Journal of Extracellular Vesicles T1 - Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients IS - 8 VL - 11 DO - 10.1002/jev2.12257 ER -
@article{ author = "Tertel, Tobias and Tomić, Sergej and Đokić, Jelena and Radojević, Dušan and Stevanović, Dejan and Ilić, Nataša and Giebel, Bernd and Kosanović, Maja", year = "2022", abstract = "COVID-19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role of extracellular vesicles (EVs) in COVID-19 and their biomarker potential, however, remains largely unknown. Aiming to identify specific EV signatures of patients with mild compared to severe COVID-19, we characterized the EV composition of 20 mild and 26 severe COVID-19 patients along with 16 sex and age-matched healthy donors with a panel of eight different antibodies by imaging flow cytometry (IFCM). We correlated the obtained data with 37 clinical, prerecorded biochemical and immunological parameters. Severe patients' sera contained increased amounts of CD13(+) and CD82(+) EVs, which positively correlated with IL-6-producing and circulating myeloid-derived suppressor cells (MDSCs) and with the serum concentration of proinflammatory cytokines, respectively. Sera of mild COVID-19 patients contained more HLA-ABC(+) EVs than sera of the healthy donors and more CD24(+) EVs than severe COVID-19 patients. Their increased abundance negatively correlated with disease severity and accumulation of MDSCs, being considered as key drivers of immunopathogenesis in COVID-19. Altogether, our results support the potential of serum EVs as powerful biomarkers for COVID-19 severity and pave the way for future investigations aiming to unravel the role of EVs in COVID-19 progression.", publisher = "Hoboken : Wiley", journal = "Journal of Extracellular Vesicles", title = "Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients", number = "8", volume = "11", doi = "10.1002/jev2.12257" }
Tertel, T., Tomić, S., Đokić, J., Radojević, D., Stevanović, D., Ilić, N., Giebel, B.,& Kosanović, M.. (2022). Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients. in Journal of Extracellular Vesicles Hoboken : Wiley., 11(8). https://doi.org/10.1002/jev2.12257
Tertel T, Tomić S, Đokić J, Radojević D, Stevanović D, Ilić N, Giebel B, Kosanović M. Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients. in Journal of Extracellular Vesicles. 2022;11(8). doi:10.1002/jev2.12257 .
Tertel, Tobias, Tomić, Sergej, Đokić, Jelena, Radojević, Dušan, Stevanović, Dejan, Ilić, Nataša, Giebel, Bernd, Kosanović, Maja, "Serum-derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID-19 patients" in Journal of Extracellular Vesicles, 11, no. 8 (2022), https://doi.org/10.1002/jev2.12257 . .