The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water
Nema prikaza
Autori
Ljubić, VericaMilosević, Milena
Cvetković, Slobodan
Stojanović, Marijana
Novović, Katarina
Dinić, Miroslav
Popović, Mina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the r...emoval efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.
Ključne reči:
Ni2+ ion sorption / L. reuteri B2 / Exopolysaccharide / Biosorbent / Adsorption capacityIzvor:
Biomass Conversion and Biorefinery, 2022Izdavač:
- Springer Heidelberg, Heidelberg
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-MESTD-inst-2020-200026)
DOI: 10.1007/s13399-022-03292-5
ISSN: 2190-6815
WoS: 000852918100001
Scopus: 2-s2.0-85137864112
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ljubić, Verica AU - Milosević, Milena AU - Cvetković, Slobodan AU - Stojanović, Marijana AU - Novović, Katarina AU - Dinić, Miroslav AU - Popović, Mina PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1523 AB - As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments. PB - Springer Heidelberg, Heidelberg T2 - Biomass Conversion and Biorefinery T1 - The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water DO - 10.1007/s13399-022-03292-5 ER -
@article{ author = "Ljubić, Verica and Milosević, Milena and Cvetković, Slobodan and Stojanović, Marijana and Novović, Katarina and Dinić, Miroslav and Popović, Mina", year = "2022", abstract = "As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.", publisher = "Springer Heidelberg, Heidelberg", journal = "Biomass Conversion and Biorefinery", title = "The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water", doi = "10.1007/s13399-022-03292-5" }
Ljubić, V., Milosević, M., Cvetković, S., Stojanović, M., Novović, K., Dinić, M.,& Popović, M.. (2022). The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water. in Biomass Conversion and Biorefinery Springer Heidelberg, Heidelberg.. https://doi.org/10.1007/s13399-022-03292-5
Ljubić V, Milosević M, Cvetković S, Stojanović M, Novović K, Dinić M, Popović M. The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water. in Biomass Conversion and Biorefinery. 2022;. doi:10.1007/s13399-022-03292-5 .
Ljubić, Verica, Milosević, Milena, Cvetković, Slobodan, Stojanović, Marijana, Novović, Katarina, Dinić, Miroslav, Popović, Mina, "The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water" in Biomass Conversion and Biorefinery (2022), https://doi.org/10.1007/s13399-022-03292-5 . .