Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production
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Stevanović, MilenaD'Agostino, Paul M.
Mojicević, Marija
Gulder, Tobias A. M.
Nikodinović-Runić, Jasmina
Vojnović, Sandra
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Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. C...onclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.
Keywords:
Streptomyces sp / staurosporine / sta cluster / elicitation / co-cultivationSource:
Journal of Applied Microbiology, 2022, 133, 4, 2560-2568Publisher:
- Wiley, Hoboken
Funding / projects:
- DAAD (Deutscher Akademischer Austauschdienst, Bilateral Project of Germany with the Republic of Serbia -2020/2021)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
DOI: 10.1111/jam.15726
ISSN: 1364-5072
PubMed: 35880373
WoS: 000834365800001
Scopus: 2-s2.0-85138462495
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stevanović, Milena AU - D'Agostino, Paul M. AU - Mojicević, Marija AU - Gulder, Tobias A. M. AU - Nikodinović-Runić, Jasmina AU - Vojnović, Sandra PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1527 AB - Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules. PB - Wiley, Hoboken T2 - Journal of Applied Microbiology T1 - Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production EP - 2568 IS - 4 SP - 2560 VL - 133 DO - 10.1111/jam.15726 ER -
@article{ author = "Stevanović, Milena and D'Agostino, Paul M. and Mojicević, Marija and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina and Vojnović, Sandra", year = "2022", abstract = "Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.", publisher = "Wiley, Hoboken", journal = "Journal of Applied Microbiology", title = "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production", pages = "2568-2560", number = "4", volume = "133", doi = "10.1111/jam.15726" }
Stevanović, M., D'Agostino, P. M., Mojicević, M., Gulder, T. A. M., Nikodinović-Runić, J.,& Vojnović, S.. (2022). Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology Wiley, Hoboken., 133(4), 2560-2568. https://doi.org/10.1111/jam.15726
Stevanović M, D'Agostino PM, Mojicević M, Gulder TAM, Nikodinović-Runić J, Vojnović S. Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology. 2022;133(4):2560-2568. doi:10.1111/jam.15726 .
Stevanović, Milena, D'Agostino, Paul M., Mojicević, Marija, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production" in Journal of Applied Microbiology, 133, no. 4 (2022):2560-2568, https://doi.org/10.1111/jam.15726 . .