Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia
2022
Autori
Buha, I.Škodrić-Trifunović, V.
Anđelković, Marina
Pavlović, Sonja
Vreća, M.
Stjepanović, M.
Milin-Lazović, Jelena
Simić, M.
Antonijević, G.
Spasovski, Vesna
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity. Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB. Results: There wa...s statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups. Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB.
Ključne reči:
miRNA-146a gene expression / Biomarker / Active pulmonary tuberculosis.Izvor:
Journal of Infection in Developing Countries, 2022, 16, 8, 1317-1332Izdavač:
- Journal of Infection in Developing Countries
Finansiranje / projekti:
- Retke bolesti: molekularna patofiziologija, dijagnostički i terapijski modaliteti i socijalni, etički i pravni aspekti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41004)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
DOI: 10.3855/jidc.16721
ISSN: 2036-6590
PubMed: 36099375
Scopus: 2-s2.0-85137762760
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Buha, I. AU - Škodrić-Trifunović, V. AU - Anđelković, Marina AU - Pavlović, Sonja AU - Vreća, M. AU - Stjepanović, M. AU - Milin-Lazović, Jelena AU - Simić, M. AU - Antonijević, G. AU - Spasovski, Vesna PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1542 AB - Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity. Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB. Results: There was statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups. Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB. PB - Journal of Infection in Developing Countries T2 - Journal of Infection in Developing Countries T1 - Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia EP - 1332 IS - 8 SP - 1317 VL - 16 DO - 10.3855/jidc.16721 ER -
@article{ author = "Buha, I. and Škodrić-Trifunović, V. and Anđelković, Marina and Pavlović, Sonja and Vreća, M. and Stjepanović, M. and Milin-Lazović, Jelena and Simić, M. and Antonijević, G. and Spasovski, Vesna", year = "2022", abstract = "Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity. Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB. Results: There was statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups. Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB.", publisher = "Journal of Infection in Developing Countries", journal = "Journal of Infection in Developing Countries", title = "Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia", pages = "1332-1317", number = "8", volume = "16", doi = "10.3855/jidc.16721" }
Buha, I., Škodrić-Trifunović, V., Anđelković, M., Pavlović, S., Vreća, M., Stjepanović, M., Milin-Lazović, J., Simić, M., Antonijević, G.,& Spasovski, V.. (2022). Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia. in Journal of Infection in Developing Countries Journal of Infection in Developing Countries., 16(8), 1317-1332. https://doi.org/10.3855/jidc.16721
Buha I, Škodrić-Trifunović V, Anđelković M, Pavlović S, Vreća M, Stjepanović M, Milin-Lazović J, Simić M, Antonijević G, Spasovski V. Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia. in Journal of Infection in Developing Countries. 2022;16(8):1317-1332. doi:10.3855/jidc.16721 .
Buha, I., Škodrić-Trifunović, V., Anđelković, Marina, Pavlović, Sonja, Vreća, M., Stjepanović, M., Milin-Lazović, Jelena, Simić, M., Antonijević, G., Spasovski, Vesna, "Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia" in Journal of Infection in Developing Countries, 16, no. 8 (2022):1317-1332, https://doi.org/10.3855/jidc.16721 . .