Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis
2022
Autori
Mandić, MarijaMitić, Katarina
Nedeljković, Predrag
Perić, Mina
Bozić, Bojan
Lunić, Tanja
Bacić, Ana
Rajilić-Stojanović, Mirjana
Peković, Sanja
Nedeljković, Biljana Bozic
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC t...reatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
Ključne reči:
vitamin B complex / popliteal lymph nodes / neuroprotection / nerve / muscle nuclear density / gut microbiota / experimental autoimmune encephalomyelitisIzvor:
Nutrients, 2022, 14, 6Izdavač:
- MDPI, Basel
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/nu14061273
ISSN: 2072-6643
PubMed: 35334928
WoS: 000774457300001
Scopus: 2-s2.0-85126446654
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mandić, Marija AU - Mitić, Katarina AU - Nedeljković, Predrag AU - Perić, Mina AU - Bozić, Bojan AU - Lunić, Tanja AU - Bacić, Ana AU - Rajilić-Stojanović, Mirjana AU - Peković, Sanja AU - Nedeljković, Biljana Bozic PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1567 AB - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE. PB - MDPI, Basel T2 - Nutrients T1 - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis IS - 6 VL - 14 DO - 10.3390/nu14061273 ER -
@article{ author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Bozić, Bojan and Lunić, Tanja and Bacić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Nedeljković, Biljana Bozic", year = "2022", abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.", publisher = "MDPI, Basel", journal = "Nutrients", title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis", number = "6", volume = "14", doi = "10.3390/nu14061273" }
Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Bozić, B., Lunić, T., Bacić, A., Rajilić-Stojanović, M., Peković, S.,& Nedeljković, B. B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients MDPI, Basel., 14(6). https://doi.org/10.3390/nu14061273
Mandić M, Mitić K, Nedeljković P, Perić M, Bozić B, Lunić T, Bacić A, Rajilić-Stojanović M, Peković S, Nedeljković BB. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients. 2022;14(6). doi:10.3390/nu14061273 .
Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Bozić, Bojan, Lunić, Tanja, Bacić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Nedeljković, Biljana Bozic, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis" in Nutrients, 14, no. 6 (2022), https://doi.org/10.3390/nu14061273 . .