Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis
Apstrakt
The BRCA2 tumor suppressor functions in repair of DNA by homologous recombination through regulating the action of Rad51. In turn, BRCA2 appears to be regulated by other interacting proteins. Dss1, a small interacting protein that binds to the C-terminal domain, has a profound effect on activity as deduced from studies on the BRCA2-related protein Brh2 in Ustilago maydis. Evidence accumulating in mammalian systems suggests that BCCIP, another small interacting protein that binds to the C-terminal domain of BRCA2, also serves to regulate homologous recombination activity. Here we were interested in testing the role of the putative U. maydis BCCIP ortholog Bcp1 in DNA repair and recombination. In keeping with the mammalian paradigm, Bcp1 bound to the C-terminal region of Brh2. Mutants deleted of the gene were extremely slow growing, showed a delay passing through S phase and exhibited sensitivity to hydroxyurea, but were other-wise normal in DNA repair and homologous recombination. In th...e absence of Bcp1 cells were unable to maintain the wild type morphology when challenged by a DNA replication stress. These results suggest that Bcp1 could be involved in coordinating morphogenetic events with DNA processing during replication.
Ključne reči:
repair / recombination / Rad51 / Dss1 / BRCA2 / BCCIPIzvor:
DNA Repair, 2007, 6, 11, 1651-1660Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- NIGMS NIH HHS [GM42482, R01 GM042482, R01 GM042482-16] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042482] Funding Source: NIH RePORTER
Napomena:
- This is the peer reviewed version of the paper: Mao, N., Zhou, Q., Kojic, M., Pérez-Martín, J., & Holloman, W. K. (2007). Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis. DNA Repair, 6(11), 1651–1660. https://doi.org/10.1016/j.dnarep.2007.05.012
- Published version: : https://imagine.imgge.bg.ac.rs/handle/123456789/278
Povezane informacije:
- Druga verzija
https://doi.org/10.1016/j.dnarep.2007.05.012 - Druga verzija
https://imagine.imgge.bg.ac.rs/handle/123456789/278
DOI: 10.1016/j.dnarep.2007.05.012
ISSN: 1568-7864
PubMed: 17627904
WoS: 000251285300009
Scopus: 2-s2.0-35348966282
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mao, Ninghui AU - Zhou, Qingwen AU - Kojić, Milorad AU - Perez-Martin, Jose AU - Holloman, William K. PY - 2007 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1616 AB - The BRCA2 tumor suppressor functions in repair of DNA by homologous recombination through regulating the action of Rad51. In turn, BRCA2 appears to be regulated by other interacting proteins. Dss1, a small interacting protein that binds to the C-terminal domain, has a profound effect on activity as deduced from studies on the BRCA2-related protein Brh2 in Ustilago maydis. Evidence accumulating in mammalian systems suggests that BCCIP, another small interacting protein that binds to the C-terminal domain of BRCA2, also serves to regulate homologous recombination activity. Here we were interested in testing the role of the putative U. maydis BCCIP ortholog Bcp1 in DNA repair and recombination. In keeping with the mammalian paradigm, Bcp1 bound to the C-terminal region of Brh2. Mutants deleted of the gene were extremely slow growing, showed a delay passing through S phase and exhibited sensitivity to hydroxyurea, but were other-wise normal in DNA repair and homologous recombination. In the absence of Bcp1 cells were unable to maintain the wild type morphology when challenged by a DNA replication stress. These results suggest that Bcp1 could be involved in coordinating morphogenetic events with DNA processing during replication. PB - Elsevier, Amsterdam T2 - DNA Repair T1 - Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis EP - 1660 IS - 11 SP - 1651 VL - 6 DO - 10.1016/j.dnarep.2007.05.012 ER -
@article{ author = "Mao, Ninghui and Zhou, Qingwen and Kojić, Milorad and Perez-Martin, Jose and Holloman, William K.", year = "2007", abstract = "The BRCA2 tumor suppressor functions in repair of DNA by homologous recombination through regulating the action of Rad51. In turn, BRCA2 appears to be regulated by other interacting proteins. Dss1, a small interacting protein that binds to the C-terminal domain, has a profound effect on activity as deduced from studies on the BRCA2-related protein Brh2 in Ustilago maydis. Evidence accumulating in mammalian systems suggests that BCCIP, another small interacting protein that binds to the C-terminal domain of BRCA2, also serves to regulate homologous recombination activity. Here we were interested in testing the role of the putative U. maydis BCCIP ortholog Bcp1 in DNA repair and recombination. In keeping with the mammalian paradigm, Bcp1 bound to the C-terminal region of Brh2. Mutants deleted of the gene were extremely slow growing, showed a delay passing through S phase and exhibited sensitivity to hydroxyurea, but were other-wise normal in DNA repair and homologous recombination. In the absence of Bcp1 cells were unable to maintain the wild type morphology when challenged by a DNA replication stress. These results suggest that Bcp1 could be involved in coordinating morphogenetic events with DNA processing during replication.", publisher = "Elsevier, Amsterdam", journal = "DNA Repair", title = "Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis", pages = "1660-1651", number = "11", volume = "6", doi = "10.1016/j.dnarep.2007.05.012" }
Mao, N., Zhou, Q., Kojić, M., Perez-Martin, J.,& Holloman, W. K.. (2007). Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis. in DNA Repair Elsevier, Amsterdam., 6(11), 1651-1660. https://doi.org/10.1016/j.dnarep.2007.05.012
Mao N, Zhou Q, Kojić M, Perez-Martin J, Holloman WK. Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis. in DNA Repair. 2007;6(11):1651-1660. doi:10.1016/j.dnarep.2007.05.012 .
Mao, Ninghui, Zhou, Qingwen, Kojić, Milorad, Perez-Martin, Jose, Holloman, William K., "Ortholog of BRCA2-interacting protein BCCIP controls morphogenetic responses during DNA replication stress in Ustilago maydis" in DNA Repair, 6, no. 11 (2007):1651-1660, https://doi.org/10.1016/j.dnarep.2007.05.012 . .