Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis
Članak u časopisu (Recenzirana verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Inactivation of the structural gene for the RecQ family member, BLM in human, Sgs1 in budding yeast, or Rqh1 in fission yeast leads to inappropriate recombination, chromosome abnormalities, and disturbed replication fork progression. Studies with yeasts have demonstrated that auxiliary gene functions can contribute in overlapping ways with Sgs1 or Rqh1 to circumvent or overcome lesions in DNA caused by certain genotoxic agents. In the combined absence of these functions, recombination-mediated processes lead to severe loss of fitness. Here we performed a genetic study to determine the role of the Ustilago maydis Blm homolog in DNA repair and in alleviating replication stress. We characterized the single mutant as well as double mutants additionally deleted of genes encoding Srs2, Fbh1, Mus81, or Exo1. Unlike yeasts, neither the blm srs2, blm exo1. nor blm mus81 double mutant exhibited extreme loss of fitness. Inactivation of Brh2, the BRCA2 homolog, suppressed toxicity to hydroxyurea c...aused by loss of Blm function. However, differential suppression by Brh2 derivatives lacking the canonical DNA-binding region suggests that the particular domain structure comprising this DNA-binding region may be instrumental in promoting the observed hydroxyurea toxicity.
Ključne reči:
Srs2 / Recombination / Rad51 / Mus81 / Fbh1 / Exo1 / DNA repair / BRCA2 / BLMIzvor:
DNA Repair, 2009, 8, 6, 752-759Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Milo Gladstein Foundation
- NIH [GM42482]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042482] Funding Source: NIH RePORTER
Napomena:
- This is the peer reviewed version of the paper: Mao, N., Kojić, M., & Holloman, W. K. (2009). Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis. DNA Repair, 8(6), 752–759. https://doi.org/10.1016/j.dnarep.2009.02.006
- Published version: https://imagine.imgge.bg.ac.rs/handle/123456789/343
Povezane informacije:
- Druga verzija
https://doi.org/10.1016/j.dnarep.2009.02.006 - Druga verzija
https://imagine.imgge.bg.ac.rs/handle/123456789/343
DOI: 10.1016/j.dnarep.2009.02.006
ISSN: 1568-7864
PubMed: 19349216
WoS: 000267159000008
Scopus: 2-s2.0-67349245485
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mao, Ninghui AU - Kojić, Milorad AU - Holloman, William K. PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1617 AB - Inactivation of the structural gene for the RecQ family member, BLM in human, Sgs1 in budding yeast, or Rqh1 in fission yeast leads to inappropriate recombination, chromosome abnormalities, and disturbed replication fork progression. Studies with yeasts have demonstrated that auxiliary gene functions can contribute in overlapping ways with Sgs1 or Rqh1 to circumvent or overcome lesions in DNA caused by certain genotoxic agents. In the combined absence of these functions, recombination-mediated processes lead to severe loss of fitness. Here we performed a genetic study to determine the role of the Ustilago maydis Blm homolog in DNA repair and in alleviating replication stress. We characterized the single mutant as well as double mutants additionally deleted of genes encoding Srs2, Fbh1, Mus81, or Exo1. Unlike yeasts, neither the blm srs2, blm exo1. nor blm mus81 double mutant exhibited extreme loss of fitness. Inactivation of Brh2, the BRCA2 homolog, suppressed toxicity to hydroxyurea caused by loss of Blm function. However, differential suppression by Brh2 derivatives lacking the canonical DNA-binding region suggests that the particular domain structure comprising this DNA-binding region may be instrumental in promoting the observed hydroxyurea toxicity. PB - Elsevier, Amsterdam T2 - DNA Repair T1 - Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis EP - 759 IS - 6 SP - 752 VL - 8 DO - 10.1016/j.dnarep.2009.02.006 ER -
@article{ author = "Mao, Ninghui and Kojić, Milorad and Holloman, William K.", year = "2009", abstract = "Inactivation of the structural gene for the RecQ family member, BLM in human, Sgs1 in budding yeast, or Rqh1 in fission yeast leads to inappropriate recombination, chromosome abnormalities, and disturbed replication fork progression. Studies with yeasts have demonstrated that auxiliary gene functions can contribute in overlapping ways with Sgs1 or Rqh1 to circumvent or overcome lesions in DNA caused by certain genotoxic agents. In the combined absence of these functions, recombination-mediated processes lead to severe loss of fitness. Here we performed a genetic study to determine the role of the Ustilago maydis Blm homolog in DNA repair and in alleviating replication stress. We characterized the single mutant as well as double mutants additionally deleted of genes encoding Srs2, Fbh1, Mus81, or Exo1. Unlike yeasts, neither the blm srs2, blm exo1. nor blm mus81 double mutant exhibited extreme loss of fitness. Inactivation of Brh2, the BRCA2 homolog, suppressed toxicity to hydroxyurea caused by loss of Blm function. However, differential suppression by Brh2 derivatives lacking the canonical DNA-binding region suggests that the particular domain structure comprising this DNA-binding region may be instrumental in promoting the observed hydroxyurea toxicity.", publisher = "Elsevier, Amsterdam", journal = "DNA Repair", title = "Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis", pages = "759-752", number = "6", volume = "8", doi = "10.1016/j.dnarep.2009.02.006" }
Mao, N., Kojić, M.,& Holloman, W. K.. (2009). Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis. in DNA Repair Elsevier, Amsterdam., 8(6), 752-759. https://doi.org/10.1016/j.dnarep.2009.02.006
Mao N, Kojić M, Holloman WK. Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis. in DNA Repair. 2009;8(6):752-759. doi:10.1016/j.dnarep.2009.02.006 .
Mao, Ninghui, Kojić, Milorad, Holloman, William K., "Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis" in DNA Repair, 8, no. 6 (2009):752-759, https://doi.org/10.1016/j.dnarep.2009.02.006 . .