Mutational analysis of Brh2 reveals requirements for compensating mediator functions
Апстракт
Brh2, a member of the BRCA2 family of proteins, governs homologous recombination in the
fungus Ustilago maydis through interaction with Rad51. Brh2 serves at an early step in
homologous recombination to mediate Rad51 nucleoprotein filament formation and also has the
capability to function at a later step in recombination through its inherent DNA annealing activity.
Rec2, a Rad51 paralog, and Rad52, are additional components of the homologous recombination
system, but the absence of either is less critical than Brh2 for operational activity. Here we tested a
variety of mutant forms of Brh2 for activity in recombinational repair as measured by DNA repair
proficiency. We found that a mutant of Brh2 deleted of the non-canonical DNA-binding domain
within the N-terminal region is dependent upon the presence of Rad52 for DNA-repair activity.
We also determined that a motif first identified in human BRCA2 as important in binding DMC1
also contributes to DNA repair proficiency and coo...perates with the BRC element in Rad51
binding.
Извор:
Molecular Microbiology, 2011, 79, 1, 180-191Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- NIH [GM042482, GM079859]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042482, R01GM079859] Funding Source: NIH RePORTER
Напомена:
- This is the peer-reviewed version of the article: Kojic, M., Zhou, Q., Fan, J., & Holloman, W. K. (2011). Mutational analysis of Brh2 reveals requirements for compensating mediator functions. Molecular Microbiology, 79(1), 180–191. https://doi.org/10.1111/j.1365-2958.2010.07440.x
- Published version: https://imagine.imgge.bg.ac.rs/handle/123456789/476
Повезане информације:
- Друга верзија
https://imagine.imgge.bg.ac.rs/handle/123456789/476 - Друга верзија
https://doi.org/10.1111/j.1365-2958.2010.07440.x
DOI: 10.1111/j.1365-2958.2010.07440.x
ISSN: 0950-382X
PubMed: 21166902
WoS: 000285762100015
Scopus: 2-s2.0-78650241099
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milorad AU - Zhou, Qingwen AU - Fan, Jie AU - Holloman, William K. PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1619 AB - Brh2, a member of the BRCA2 family of proteins, governs homologous recombination in the fungus Ustilago maydis through interaction with Rad51. Brh2 serves at an early step in homologous recombination to mediate Rad51 nucleoprotein filament formation and also has the capability to function at a later step in recombination through its inherent DNA annealing activity. Rec2, a Rad51 paralog, and Rad52, are additional components of the homologous recombination system, but the absence of either is less critical than Brh2 for operational activity. Here we tested a variety of mutant forms of Brh2 for activity in recombinational repair as measured by DNA repair proficiency. We found that a mutant of Brh2 deleted of the non-canonical DNA-binding domain within the N-terminal region is dependent upon the presence of Rad52 for DNA-repair activity. We also determined that a motif first identified in human BRCA2 as important in binding DMC1 also contributes to DNA repair proficiency and cooperates with the BRC element in Rad51 binding. PB - Wiley, Hoboken T2 - Molecular Microbiology T1 - Mutational analysis of Brh2 reveals requirements for compensating mediator functions EP - 191 IS - 1 SP - 180 VL - 79 DO - 10.1111/j.1365-2958.2010.07440.x ER -
@article{ author = "Kojić, Milorad and Zhou, Qingwen and Fan, Jie and Holloman, William K.", year = "2011", abstract = "Brh2, a member of the BRCA2 family of proteins, governs homologous recombination in the fungus Ustilago maydis through interaction with Rad51. Brh2 serves at an early step in homologous recombination to mediate Rad51 nucleoprotein filament formation and also has the capability to function at a later step in recombination through its inherent DNA annealing activity. Rec2, a Rad51 paralog, and Rad52, are additional components of the homologous recombination system, but the absence of either is less critical than Brh2 for operational activity. Here we tested a variety of mutant forms of Brh2 for activity in recombinational repair as measured by DNA repair proficiency. We found that a mutant of Brh2 deleted of the non-canonical DNA-binding domain within the N-terminal region is dependent upon the presence of Rad52 for DNA-repair activity. We also determined that a motif first identified in human BRCA2 as important in binding DMC1 also contributes to DNA repair proficiency and cooperates with the BRC element in Rad51 binding.", publisher = "Wiley, Hoboken", journal = "Molecular Microbiology", title = "Mutational analysis of Brh2 reveals requirements for compensating mediator functions", pages = "191-180", number = "1", volume = "79", doi = "10.1111/j.1365-2958.2010.07440.x" }
Kojić, M., Zhou, Q., Fan, J.,& Holloman, W. K.. (2011). Mutational analysis of Brh2 reveals requirements for compensating mediator functions. in Molecular Microbiology Wiley, Hoboken., 79(1), 180-191. https://doi.org/10.1111/j.1365-2958.2010.07440.x
Kojić M, Zhou Q, Fan J, Holloman WK. Mutational analysis of Brh2 reveals requirements for compensating mediator functions. in Molecular Microbiology. 2011;79(1):180-191. doi:10.1111/j.1365-2958.2010.07440.x .
Kojić, Milorad, Zhou, Qingwen, Fan, Jie, Holloman, William K., "Mutational analysis of Brh2 reveals requirements for compensating mediator functions" in Molecular Microbiology, 79, no. 1 (2011):180-191, https://doi.org/10.1111/j.1365-2958.2010.07440.x . .