Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics
Autori
Nikolaivits, EfstratiosDimopoulou, Phaedra
Maslak, Veselin
Nikodinović-Runić, Jasmina
Topakas, Evangelos
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Plastic waste poses an enormous environmental problem as a result of soil and ocean contamination, causing the release of microplastics that end up in humans through the food web. Enzymatic degradation of plastics has emerged as an alternative to traditional recycling processes. In the present work, we used bioinfomatics tools to discover a gene coding for a putative polyester degrading enzyme (polyesterase). The gene was heterologously expressed, purified and biochemically characterized. Furthermore, its ability to degrade polyethylene terephthalate (PET) model substrates and synthetic plastics was assessed.
Ključne reči:
novel enzyme / plastics / polymer degradationIzvor:
Chemistry Proceedings, 2020, 2, 1, 33-Povezane informacije:
- Druga verzija
https://www.mdpi.com/2673-4583/2/1/33 - Druga verzija
https://doi.org/10.3390/ECCS2020-07572
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Nikolaivits, Efstratios AU - Dimopoulou, Phaedra AU - Maslak, Veselin AU - Nikodinović-Runić, Jasmina AU - Topakas, Evangelos PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1633 AB - Plastic waste poses an enormous environmental problem as a result of soil and ocean contamination, causing the release of microplastics that end up in humans through the food web. Enzymatic degradation of plastics has emerged as an alternative to traditional recycling processes. In the present work, we used bioinfomatics tools to discover a gene coding for a putative polyester degrading enzyme (polyesterase). The gene was heterologously expressed, purified and biochemically characterized. Furthermore, its ability to degrade polyethylene terephthalate (PET) model substrates and synthetic plastics was assessed. T2 - Chemistry Proceedings T2 - Chemistry Proceedings T1 - Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics IS - 1 SP - 33 VL - 2 DO - 10.3390/ECCS2020-07572 ER -
@article{ author = "Nikolaivits, Efstratios and Dimopoulou, Phaedra and Maslak, Veselin and Nikodinović-Runić, Jasmina and Topakas, Evangelos", year = "2020", abstract = "Plastic waste poses an enormous environmental problem as a result of soil and ocean contamination, causing the release of microplastics that end up in humans through the food web. Enzymatic degradation of plastics has emerged as an alternative to traditional recycling processes. In the present work, we used bioinfomatics tools to discover a gene coding for a putative polyester degrading enzyme (polyesterase). The gene was heterologously expressed, purified and biochemically characterized. Furthermore, its ability to degrade polyethylene terephthalate (PET) model substrates and synthetic plastics was assessed.", journal = "Chemistry Proceedings, Chemistry Proceedings", title = "Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics", number = "1", pages = "33", volume = "2", doi = "10.3390/ECCS2020-07572" }
Nikolaivits, E., Dimopoulou, P., Maslak, V., Nikodinović-Runić, J.,& Topakas, E.. (2020). Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics. in Chemistry Proceedings, 2(1), 33. https://doi.org/10.3390/ECCS2020-07572
Nikolaivits E, Dimopoulou P, Maslak V, Nikodinović-Runić J, Topakas E. Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics. in Chemistry Proceedings. 2020;2(1):33. doi:10.3390/ECCS2020-07572 .
Nikolaivits, Efstratios, Dimopoulou, Phaedra, Maslak, Veselin, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics" in Chemistry Proceedings, 2, no. 1 (2020):33, https://doi.org/10.3390/ECCS2020-07572 . .