Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype
Апстракт
The cysB gene product is a LysR-type regulatory protein required for expression of the cys regulon. cysB mutants of Escherichia coli and Salmonella, along with being auxotrophs for the cysteine, exhibit increased resistance to the antibiotics novobiocin (Nov) and mecillinam. In this work, by using lambdaplacMu9 insertions creating random lacZ fusions, we identify a gene, hslJ, whose expression appeared to be increased in cysB mutants and needed for Nov resistance. Measurements of the hslJ::IacZ gene fusion expression demonstrated that the hslJ gene is negatively regulated by CysB. In addition we observe the negative autogenous control of HslJ. When the control imposed by CysB is lifted in the cysB mutant, the elevation of Nov resistance can be achieved only in the presence of wild-type hslJ allele. A double cysB hslJ mutant restores the sensitivity to Nov. Overexpression of the wild-type HslJ protein either in a cysB(+) or a cys(B-) background increases the level of Nov resistance indi...cating that hslJ product is indeed involved in accomplishing this phenotype. The hslJ::OmegaKan allele encodes the C-terminaly truncated mutant protein HslJ Q121Ter which is not functional in achieving the Nov resistance but when overexpressed induces the psp operon. Finally, we found that inactivation of hslJ does not affect the increased resistance to mecillinam in cysB mutants.
Кључне речи:
resistance to novobiocin / negative regulation / hslJ / cysBИзвор:
FEMS Microbiology Letters, 2003, 224, 2, 239-246Издавач:
- Oxford Univ Press, Oxford
DOI: 10.1016/S0378-1097(03)00441-5
ISSN: 0378-1097
PubMed: 12892888
WoS: 000184569700012
Scopus: 2-s2.0-0043210397
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Lilić, M AU - Jovanović, Milija AU - Jovanović, Goran AU - Savić, DJ PY - 2003 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/176 AB - The cysB gene product is a LysR-type regulatory protein required for expression of the cys regulon. cysB mutants of Escherichia coli and Salmonella, along with being auxotrophs for the cysteine, exhibit increased resistance to the antibiotics novobiocin (Nov) and mecillinam. In this work, by using lambdaplacMu9 insertions creating random lacZ fusions, we identify a gene, hslJ, whose expression appeared to be increased in cysB mutants and needed for Nov resistance. Measurements of the hslJ::IacZ gene fusion expression demonstrated that the hslJ gene is negatively regulated by CysB. In addition we observe the negative autogenous control of HslJ. When the control imposed by CysB is lifted in the cysB mutant, the elevation of Nov resistance can be achieved only in the presence of wild-type hslJ allele. A double cysB hslJ mutant restores the sensitivity to Nov. Overexpression of the wild-type HslJ protein either in a cysB(+) or a cys(B-) background increases the level of Nov resistance indicating that hslJ product is indeed involved in accomplishing this phenotype. The hslJ::OmegaKan allele encodes the C-terminaly truncated mutant protein HslJ Q121Ter which is not functional in achieving the Nov resistance but when overexpressed induces the psp operon. Finally, we found that inactivation of hslJ does not affect the increased resistance to mecillinam in cysB mutants. PB - Oxford Univ Press, Oxford T2 - FEMS Microbiology Letters T1 - Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype EP - 246 IS - 2 SP - 239 VL - 224 DO - 10.1016/S0378-1097(03)00441-5 ER -
@article{ author = "Lilić, M and Jovanović, Milija and Jovanović, Goran and Savić, DJ", year = "2003", abstract = "The cysB gene product is a LysR-type regulatory protein required for expression of the cys regulon. cysB mutants of Escherichia coli and Salmonella, along with being auxotrophs for the cysteine, exhibit increased resistance to the antibiotics novobiocin (Nov) and mecillinam. In this work, by using lambdaplacMu9 insertions creating random lacZ fusions, we identify a gene, hslJ, whose expression appeared to be increased in cysB mutants and needed for Nov resistance. Measurements of the hslJ::IacZ gene fusion expression demonstrated that the hslJ gene is negatively regulated by CysB. In addition we observe the negative autogenous control of HslJ. When the control imposed by CysB is lifted in the cysB mutant, the elevation of Nov resistance can be achieved only in the presence of wild-type hslJ allele. A double cysB hslJ mutant restores the sensitivity to Nov. Overexpression of the wild-type HslJ protein either in a cysB(+) or a cys(B-) background increases the level of Nov resistance indicating that hslJ product is indeed involved in accomplishing this phenotype. The hslJ::OmegaKan allele encodes the C-terminaly truncated mutant protein HslJ Q121Ter which is not functional in achieving the Nov resistance but when overexpressed induces the psp operon. Finally, we found that inactivation of hslJ does not affect the increased resistance to mecillinam in cysB mutants.", publisher = "Oxford Univ Press, Oxford", journal = "FEMS Microbiology Letters", title = "Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype", pages = "246-239", number = "2", volume = "224", doi = "10.1016/S0378-1097(03)00441-5" }
Lilić, M., Jovanović, M., Jovanović, G.,& Savić, D.. (2003). Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype. in FEMS Microbiology Letters Oxford Univ Press, Oxford., 224(2), 239-246. https://doi.org/10.1016/S0378-1097(03)00441-5
Lilić M, Jovanović M, Jovanović G, Savić D. Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype. in FEMS Microbiology Letters. 2003;224(2):239-246. doi:10.1016/S0378-1097(03)00441-5 .
Lilić, M, Jovanović, Milija, Jovanović, Goran, Savić, DJ, "Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype" in FEMS Microbiology Letters, 224, no. 2 (2003):239-246, https://doi.org/10.1016/S0378-1097(03)00441-5 . .