Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
Samo za registrovane korisnike
2022
Autori
Stevanović, MagdalenaFilipović, Nenad
Kuzmanović, Maja
Tomić, Nina
Ušjak, Dušan
Milenković, Marina
Zheng, Kai
Stampfl, Juergen
Boccaccin, Aldo
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue
engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an
element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the
incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to
produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively
characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry
(ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission
scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as
collagen/SeNPs, was tested on the MRC-5 ce...lls. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect.
Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents
of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP)
scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by
newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs
were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs
was significantly reduced compared to the uncoated ones.
Ključne reči:
biomaterials / composite materials / selenium nanoparticles / collagen / antimicrobial activity / scaffoldsIzvor:
Nanotechnology in Biomaterials, 2022, 36, 10Izdavač:
- SAGE
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200175 (Institut tehničkih nauka SANU, Beograd) (RS-MESTD-inst-2020-200175)
- The bilateral project between Serbia and Austria (project No: SRB 24/2018, project title: Scaffolds with controlled 3-D architecture designed by photopolymerization),
- The bilateral project between Serbia and Germany (DAAD project 57514776)
Napomena:
- Supplementary material: https://imagine.imgge.bg.ac.rs/handle/123456789/1774
DOI: 10.1177/08853282211073731
ISSN: 0885-3282; 1530-8022 (Online)
WoS: 000764244600001
Scopus: 2-s2.0-85126034982
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stevanović, Magdalena AU - Filipović, Nenad AU - Kuzmanović, Maja AU - Tomić, Nina AU - Ušjak, Dušan AU - Milenković, Marina AU - Zheng, Kai AU - Stampfl, Juergen AU - Boccaccin, Aldo PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1773 AB - Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. PB - SAGE T2 - Nanotechnology in Biomaterials T1 - Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles IS - 10 VL - 36 DO - 10.1177/08853282211073731 ER -
@article{ author = "Stevanović, Magdalena and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccin, Aldo", year = "2022", abstract = "Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones.", publisher = "SAGE", journal = "Nanotechnology in Biomaterials", title = "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles", number = "10", volume = "36", doi = "10.1177/08853282211073731" }
Stevanović, M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccin, A.. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Nanotechnology in Biomaterials SAGE., 36(10). https://doi.org/10.1177/08853282211073731
Stevanović M, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccin A. Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Nanotechnology in Biomaterials. 2022;36(10). doi:10.1177/08853282211073731 .
Stevanović, Magdalena, Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccin, Aldo, "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles" in Nanotechnology in Biomaterials, 36, no. 10 (2022), https://doi.org/10.1177/08853282211073731 . .