Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes
Само за регистроване кориснике
2023
Аутори
Taxeidis, GeorgeNikolaivits, Efstratios
Siaperas, Romanos
Gkountela, Christina
Vouyiouka, Stamatina
Pantelić, Brana
Nikodinović-Runić, Jasmina
Topakas, Evangelos
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforeme...ntioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.
Кључне речи:
Enzyme / Fungus / Microbial degradation / Plastics / Polyethylene / PolyurethaneИзвор:
Environmental Pollution, 2023, 325, 121460-Финансирање / пројекти:
- This research was funded by European Union’s Horizon 2020 research and innovation programme under grant agreement No 870292 (BioICEP Project)
- National Natural Science Foundation of China (Nos. 31961133016, 31961133015, and 31961133014)
- Hellenic Foundation for Research and Innovation (H⋅F.R.I.) under the “2nd Call for H⋅F.R.I. Research Projects to support Faculty Members and Researchers” (Project Number: 03061)
- The work of George Taxeidis was supported financially by the H.F.R.I (Elidek) institution (PhD Scholarship).
Напомена:
- The authors would like to thank the VIB Proteomics Core for the contribution regarding the mass spectrometry-based proteomics experiments (EPIC-XS, project number 823839, funded by the Horizon 2020 programme of the European Union).
- Related to accepted version: https://imagine.imgge.bg.ac.rs/handle/123456789/1800
- Related to other materials: https://zenodo.org/record/7767083#.ZCP8WXZBxPa
Повезане информације:
- Друга верзија
https://imagine.imgge.bg.ac.rs/handle/123456789/1800 - Друга верзија
https://doi.org/10.1016/j.envpol.2023.121460
URI
https://www.sciencedirect.com/science/article/pii/S0269749123004621https://imagine.imgge.bg.ac.rs/handle/123456789/1797
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Taxeidis, George AU - Nikolaivits, Efstratios AU - Siaperas, Romanos AU - Gkountela, Christina AU - Vouyiouka, Stamatina AU - Pantelić, Brana AU - Nikodinović-Runić, Jasmina AU - Topakas, Evangelos PY - 2023 UR - https://www.sciencedirect.com/science/article/pii/S0269749123004621 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1797 AB - The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification. T2 - Environmental Pollution T1 - Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes SP - 121460 VL - 325 DO - 10.1016/j.envpol.2023.121460 ER -
@article{ author = "Taxeidis, George and Nikolaivits, Efstratios and Siaperas, Romanos and Gkountela, Christina and Vouyiouka, Stamatina and Pantelić, Brana and Nikodinović-Runić, Jasmina and Topakas, Evangelos", year = "2023", abstract = "The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.", journal = "Environmental Pollution", title = "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes", pages = "121460", volume = "325", doi = "10.1016/j.envpol.2023.121460" }
Taxeidis, G., Nikolaivits, E., Siaperas, R., Gkountela, C., Vouyiouka, S., Pantelić, B., Nikodinović-Runić, J.,& Topakas, E.. (2023). Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution, 325, 121460. https://doi.org/10.1016/j.envpol.2023.121460
Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelić B, Nikodinović-Runić J, Topakas E. Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution. 2023;325:121460. doi:10.1016/j.envpol.2023.121460 .
Taxeidis, George, Nikolaivits, Efstratios, Siaperas, Romanos, Gkountela, Christina, Vouyiouka, Stamatina, Pantelić, Brana, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes" in Environmental Pollution, 325 (2023):121460, https://doi.org/10.1016/j.envpol.2023.121460 . .