Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds
Само за регистроване кориснике
2003
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The pepstatin A sensitive acidic proteolytic activity of total protein extracts of buckwheat seeds has been analyzed in developing, mature, and germinating seeds by activity measurements as well as by electrophoretic and immunochemical techniques. Immunoblot analysis using cross-reactive antibodies raised against barley phytepsin suggested that specific proteolytic activity could be attributed to a 47 kDa heterodimeric polypeptide, composed of two subunits: 31 and 16 kDa polypeptides. The analysis of time course expression revealed that the 47 kDa heterodimer accumulated during seed maturation starting from 12 days after pollination and was also present at the beginning of germination. Milk-clotting activity of this proteinase was also indicated.
Кључне речи:
milk-clotting activity / immunodetection / buckwheat / aspartic proteinase / "in-gel" assayИзвор:
Journal of Agricultural and Food Chemistry, 2003, 51, 7, 2100-2104Издавач:
- Amer Chemical Soc, Washington
DOI: 10.1021/jf026043b
ISSN: 0021-8561
PubMed: 12643680
WoS: 000181706300055
Scopus: 2-s2.0-0242669343
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Timotijević, Gordana AU - Radović, SR AU - Maksimović, Vesna R. PY - 2003 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/183 AB - The pepstatin A sensitive acidic proteolytic activity of total protein extracts of buckwheat seeds has been analyzed in developing, mature, and germinating seeds by activity measurements as well as by electrophoretic and immunochemical techniques. Immunoblot analysis using cross-reactive antibodies raised against barley phytepsin suggested that specific proteolytic activity could be attributed to a 47 kDa heterodimeric polypeptide, composed of two subunits: 31 and 16 kDa polypeptides. The analysis of time course expression revealed that the 47 kDa heterodimer accumulated during seed maturation starting from 12 days after pollination and was also present at the beginning of germination. Milk-clotting activity of this proteinase was also indicated. PB - Amer Chemical Soc, Washington T2 - Journal of Agricultural and Food Chemistry T1 - Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds EP - 2104 IS - 7 SP - 2100 VL - 51 DO - 10.1021/jf026043b ER -
@article{ author = "Timotijević, Gordana and Radović, SR and Maksimović, Vesna R.", year = "2003", abstract = "The pepstatin A sensitive acidic proteolytic activity of total protein extracts of buckwheat seeds has been analyzed in developing, mature, and germinating seeds by activity measurements as well as by electrophoretic and immunochemical techniques. Immunoblot analysis using cross-reactive antibodies raised against barley phytepsin suggested that specific proteolytic activity could be attributed to a 47 kDa heterodimeric polypeptide, composed of two subunits: 31 and 16 kDa polypeptides. The analysis of time course expression revealed that the 47 kDa heterodimer accumulated during seed maturation starting from 12 days after pollination and was also present at the beginning of germination. Milk-clotting activity of this proteinase was also indicated.", publisher = "Amer Chemical Soc, Washington", journal = "Journal of Agricultural and Food Chemistry", title = "Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds", pages = "2104-2100", number = "7", volume = "51", doi = "10.1021/jf026043b" }
Timotijević, G., Radović, S.,& Maksimović, V. R.. (2003). Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds. in Journal of Agricultural and Food Chemistry Amer Chemical Soc, Washington., 51(7), 2100-2104. https://doi.org/10.1021/jf026043b
Timotijević G, Radović S, Maksimović VR. Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds. in Journal of Agricultural and Food Chemistry. 2003;51(7):2100-2104. doi:10.1021/jf026043b .
Timotijević, Gordana, Radović, SR, Maksimović, Vesna R., "Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds" in Journal of Agricultural and Food Chemistry, 51, no. 7 (2003):2100-2104, https://doi.org/10.1021/jf026043b . .