Electronic cigarette liquids show toxic effects in vitro and in vivo
Само за регистроване кориснике
2019
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The use of electronic cigarettes is on the rise with a public perceiving them as a safer alternative to conventional cigarettes. However, their safety has only recently started being studied to a greater extent.
The aim of this study was to test the toxicity of commercially available electronic cigarette liquids (ECL) with and without nicotine in vitro and in vivo.
Two cell lines originating from the lung tissue were used - A549 and BEAS-2B. Using MTT assay, we have shown that there is a dose dependant decrease in cell viability in case of both tested liquids, and in both cell lines. We also detected cell-free mitochondrial DNA in culture medium of treated cells, indicating necrotic cell death. In order to investigate if cells undergone necroptosis we used pre-treatment with necrostatin-1 (Nec-1), a specific RIP-1 kinase inhibitor. The addition of Nec-1 did not affect cell survival under tested conditions suggesting that the cells do not go through the process of controlled necro...sis.
In vivo toxicity of different concentrations of ECL was assessed by the analysis of survival, hatching and malformations of zebrafish embryos. The effects of ECL with nicotine were comparable to those when embryos were treated with pure nicotine at the same concentration, while the ECL without nicotine did not have toxic effects in used concentrations. The embryo mortality was increased when treated with ECL with 20µg/mL of nicotine and several embryo malformations (pericardial oedema, body curvature, shortening of the body and decreased movements) were present at 10µg/mL concentration.
Our result highlight the need for further testing the molecular effects and safety of electronic cigarettes.
Кључне речи:
chronic diseases / smoking / epithelial cellИзвор:
European Respiratory Journal, 2019, 54, PA2444-Издавач:
- The European Respiratory Society
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Ljujić, Mila AU - Vignjević, Nataša AU - Divac Rankov, Aleksandra PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1830 AB - The use of electronic cigarettes is on the rise with a public perceiving them as a safer alternative to conventional cigarettes. However, their safety has only recently started being studied to a greater extent. The aim of this study was to test the toxicity of commercially available electronic cigarette liquids (ECL) with and without nicotine in vitro and in vivo. Two cell lines originating from the lung tissue were used - A549 and BEAS-2B. Using MTT assay, we have shown that there is a dose dependant decrease in cell viability in case of both tested liquids, and in both cell lines. We also detected cell-free mitochondrial DNA in culture medium of treated cells, indicating necrotic cell death. In order to investigate if cells undergone necroptosis we used pre-treatment with necrostatin-1 (Nec-1), a specific RIP-1 kinase inhibitor. The addition of Nec-1 did not affect cell survival under tested conditions suggesting that the cells do not go through the process of controlled necrosis. In vivo toxicity of different concentrations of ECL was assessed by the analysis of survival, hatching and malformations of zebrafish embryos. The effects of ECL with nicotine were comparable to those when embryos were treated with pure nicotine at the same concentration, while the ECL without nicotine did not have toxic effects in used concentrations. The embryo mortality was increased when treated with ECL with 20µg/mL of nicotine and several embryo malformations (pericardial oedema, body curvature, shortening of the body and decreased movements) were present at 10µg/mL concentration. Our result highlight the need for further testing the molecular effects and safety of electronic cigarettes. PB - The European Respiratory Society C3 - European Respiratory Journal T1 - Electronic cigarette liquids show toxic effects in vitro and in vivo SP - PA2444 VL - 54 DO - 10.1183/13993003.congress-2019.PA2444 ER -
@conference{ author = "Ljujić, Mila and Vignjević, Nataša and Divac Rankov, Aleksandra", year = "2019", abstract = "The use of electronic cigarettes is on the rise with a public perceiving them as a safer alternative to conventional cigarettes. However, their safety has only recently started being studied to a greater extent. The aim of this study was to test the toxicity of commercially available electronic cigarette liquids (ECL) with and without nicotine in vitro and in vivo. Two cell lines originating from the lung tissue were used - A549 and BEAS-2B. Using MTT assay, we have shown that there is a dose dependant decrease in cell viability in case of both tested liquids, and in both cell lines. We also detected cell-free mitochondrial DNA in culture medium of treated cells, indicating necrotic cell death. In order to investigate if cells undergone necroptosis we used pre-treatment with necrostatin-1 (Nec-1), a specific RIP-1 kinase inhibitor. The addition of Nec-1 did not affect cell survival under tested conditions suggesting that the cells do not go through the process of controlled necrosis. In vivo toxicity of different concentrations of ECL was assessed by the analysis of survival, hatching and malformations of zebrafish embryos. The effects of ECL with nicotine were comparable to those when embryos were treated with pure nicotine at the same concentration, while the ECL without nicotine did not have toxic effects in used concentrations. The embryo mortality was increased when treated with ECL with 20µg/mL of nicotine and several embryo malformations (pericardial oedema, body curvature, shortening of the body and decreased movements) were present at 10µg/mL concentration. Our result highlight the need for further testing the molecular effects and safety of electronic cigarettes.", publisher = "The European Respiratory Society", journal = "European Respiratory Journal", title = "Electronic cigarette liquids show toxic effects in vitro and in vivo", pages = "PA2444", volume = "54", doi = "10.1183/13993003.congress-2019.PA2444" }
Ljujić, M., Vignjević, N.,& Divac Rankov, A.. (2019). Electronic cigarette liquids show toxic effects in vitro and in vivo. in European Respiratory Journal The European Respiratory Society., 54, PA2444. https://doi.org/10.1183/13993003.congress-2019.PA2444
Ljujić M, Vignjević N, Divac Rankov A. Electronic cigarette liquids show toxic effects in vitro and in vivo. in European Respiratory Journal. 2019;54:PA2444. doi:10.1183/13993003.congress-2019.PA2444 .
Ljujić, Mila, Vignjević, Nataša, Divac Rankov, Aleksandra, "Electronic cigarette liquids show toxic effects in vitro and in vivo" in European Respiratory Journal, 54 (2019):PA2444, https://doi.org/10.1183/13993003.congress-2019.PA2444 . .