Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
Аутори
Vidović, MarijaBattisti, Ilaria
Pantelić, Ana
Morina, Filis
Arrigoni, Giorgio
Masi, Antonio
Veljović Jovanović, Sonja
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear ...photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.
Кључне речи:
cell wall remodelling / cyclic electron transport / germin-like proteins / late embryogenesis abundant proteins / polyphenol oxidaseИзвор:
4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022, 27-27Финансирање / пројекти:
- LEAPSyn-SCI - Late Embryogenesis Abundant Proteins: Structural Characterisation and Interaction With Α-Synuclein (RS-ScienceFundRS-Promis-6039663)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Vidović, Marija AU - Battisti, Ilaria AU - Pantelić, Ana AU - Morina, Filis AU - Arrigoni, Giorgio AU - Masi, Antonio AU - Veljović Jovanović, Sonja PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1833 AB - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels. C3 - 4th International Conference on Plant Biology and 23rd SPPS Meeting T1 - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects EP - 27 SP - 27 UR - https://hdl.handle.net/21.15107/rcub_imagine_1833 ER -
@conference{ author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja", year = "2022", abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.", journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting", title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects", pages = "27-27", url = "https://hdl.handle.net/21.15107/rcub_imagine_1833" }
Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 27-27. https://hdl.handle.net/21.15107/rcub_imagine_1833
Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:27-27. https://hdl.handle.net/21.15107/rcub_imagine_1833 .
Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):27-27, https://hdl.handle.net/21.15107/rcub_imagine_1833 .