Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization
Аутори
Pantelić, AnaStevanović, Strahinja
Milić, Dejana
Milić Komić, Sonja
Kilibarda, Nataša
Vidović, Marija
Остала ауторства
Milutinović, MilicaКонференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period
and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection
species is the accumulation of protective late embryogenesis abundant proteins (LEAPs).
These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and
membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression
levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate
the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL
were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5,
seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties,
motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated
that R. serbica LEAP...s greatly differed among the LEA family groups. The most abundant LEA2
proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity
to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form
amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered
DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind
DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular
proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them
to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of
LEAPs are discussed with significant implications on drought tolerance improvement of crops
grown in arid areas.
Кључне речи:
3D protein structure modelling / de novo transcriptome assembly / intrinsically disordered proteins / liquid-liquid phase separation / secondary structure predictionИзвор:
4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022, 95-Издавач:
- Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
Финансирање / пројекти:
- This research was funded by the Science Fund of the Republic of Serbia-RS (PROMIS project LEAPSyn-SCI, grant no. 6039663)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Pantelić, Ana AU - Stevanović, Strahinja AU - Milić, Dejana AU - Milić Komić, Sonja AU - Kilibarda, Nataša AU - Vidović, Marija PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1846 AB - An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection species is the accumulation of protective late embryogenesis abundant proteins (LEAPs). These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5, seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties, motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2 proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of LEAPs are discussed with significant implications on drought tolerance improvement of crops grown in arid areas. PB - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade C3 - 4th International Conference on Plant Biology and 23rd SPPS Meeting T1 - Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization SP - 95 UR - https://hdl.handle.net/21.15107/rcub_imagine_1846 ER -
@conference{ author = "Pantelić, Ana and Stevanović, Strahinja and Milić, Dejana and Milić Komić, Sonja and Kilibarda, Nataša and Vidović, Marija", year = "2022", abstract = "An ancient resurrection plant Ramonda serbica Panc. is able to survive a long desiccation period and reestablish metabolic activity upon watering. A hallmark of desiccation tolerance in the resurrection species is the accumulation of protective late embryogenesis abundant proteins (LEAPs). These intrinsically disordered proteins (IDPs) may stabilize the correct structure of proteins and membranes during cellular dehydration. The aim of our study was to assess LEA genes’ expression levels in hydrated (HL) and desiccated leaves (DL) and to identify, characterise, and estimate the potential role of R. serbica LEAPs in desiccation tolerance. In total, 318 LEAPs from HL and DL were identified and classified into the seven LEA protein family groups ranging from LEA1-LEA5, seed maturation proteins (SMPs), and dehydrins (DEH). Analysis of the physicochemical properties, motif architecture, secondary structure, homology, and phylogenetic relationships demonstrated that R. serbica LEAPs greatly differed among the LEA family groups. The most abundant LEA2 proteins (mostly downregulated upon desiccation) exhibited lower hydrophilicity and propensity to fold into organised globular domains. Oppositely, hydrophilic LEA4 proteins tended to form amphipathic, A-type, α-helices. Most of desiccation-upregulated LEA genes encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other ID LEAPs (e.g. LEA1, LEA3, LEA4) might participate in forming intracellular proteinaceous condensates or adopt amphipathic α-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. Taken together, possible functions of LEAPs are discussed with significant implications on drought tolerance improvement of crops grown in arid areas.", publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade", journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting", title = "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization", pages = "95", url = "https://hdl.handle.net/21.15107/rcub_imagine_1846" }
Pantelić, A., Stevanović, S., Milić, D., Milić Komić, S., Kilibarda, N.,& Vidović, M.. (2022). Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in 4th International Conference on Plant Biology and 23rd SPPS Meeting Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 95. https://hdl.handle.net/21.15107/rcub_imagine_1846
Pantelić A, Stevanović S, Milić D, Milić Komić S, Kilibarda N, Vidović M. Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:95. https://hdl.handle.net/21.15107/rcub_imagine_1846 .
Pantelić, Ana, Stevanović, Strahinja, Milić, Dejana, Milić Komić, Sonja, Kilibarda, Nataša, Vidović, Marija, "Late embryogenesis abundant (LEA) proteins in Ramonda serbica Panc identification, classification and structural characterization" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):95, https://hdl.handle.net/21.15107/rcub_imagine_1846 .