Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
Аутори
Vidović, MarijaBattisti, Ilaria
Pantelić, Ana
Morina, Filis
Arrigoni, Giorgio
Masi, Antonio
Veljović Jovanović, Sonja
Остала ауторства
Milutinović, MilicaКонференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shi...fting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.
Кључне речи:
cell wall remodelling / cyclic electron transport / germin-like proteins / late embryogenesis abundant proteins / polyphenol oxidaseИзвор:
4th International Conference on Plant Biology and 23rd SPPS Meeting, 2022, 57-Издавач:
- Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
Финансирање / пројекти:
- This research was funded by the Science Fund of the Republic of Serbia-RS (PROMIS project LEAPSyn-SCI, grant no. 6039663)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Vidović, Marija AU - Battisti, Ilaria AU - Pantelić, Ana AU - Morina, Filis AU - Arrigoni, Giorgio AU - Masi, Antonio AU - Veljović Jovanović, Sonja PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1847 AB - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long period and fully resume metabolic functions upon rewatering in a very short period, even within 48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics, proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain were performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significant difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the majority of proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/ HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels. PB - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade C3 - 4th International Conference on Plant Biology and 23rd SPPS Meeting T1 - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects SP - 57 UR - https://hdl.handle.net/21.15107/rcub_imagine_1847 ER -
@conference{ author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja", year = "2022", abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long period and fully resume metabolic functions upon rewatering in a very short period, even within 48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics, proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain were performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significant difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the majority of proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/ HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.", publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade", journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting", title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects", pages = "57", url = "https://hdl.handle.net/21.15107/rcub_imagine_1847" }
Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 57. https://hdl.handle.net/21.15107/rcub_imagine_1847
Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:57. https://hdl.handle.net/21.15107/rcub_imagine_1847 .
Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):57, https://hdl.handle.net/21.15107/rcub_imagine_1847 .