Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation tolerance in Ramonda serbica Panc.
Аутори
Vidović, MarijaBattisti, Ilaria
Pantelić, Ana
Morina, Filis
Arrigoni, Giorgio
Masi, Antonio
Veljović Jovanović, Sonja
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET
to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn ...superoxide dismutase
(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●
activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.
Кључне речи:
cell wall remodelling / drought / germin-like proteins / late embryogenesis abundant proteins / resurrection plantИзвор:
5th International Conference on Plant Proteomics, 2023Издавач:
- International Plant Proteomics Organization (INPPO)
Финансирање / пројекти:
- Acknowledgement: This work was supported by the Science Fund of the Republic of Serbia (PROMIS project LEAPSyn-SCI, grant number 6039663)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- M.V. wishes to acknowledge the support of COST Action BM1405 for approving STSM in Padua during 2017 and 2018
Напомена:
- Abstract: 5th Conference of the International Plant Proteomics Organization; May 22-25, 2022 | Porto Palace Hotel | Thessaloniki, Hellas
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Vidović, Marija AU - Battisti, Ilaria AU - Pantelić, Ana AU - Morina, Filis AU - Arrigoni, Giorgio AU - Masi, Antonio AU - Veljović Jovanović, Sonja PY - 2023 UR - https://thessinppo2023.com/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1850 AB - Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and fully restores its metabolic functions just one day after watering. The aim of this study was to identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance. We combined differential transcriptomics and proteomics with the analysis of phenolics, sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins and transcripts related to photosynthetic processes were downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate- glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall remodelling related to GLP-derived H2O2/HO● activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of important metabolic pathways during desiccation with the final aim to help improving the drought tolerance in crops. PB - International Plant Proteomics Organization (INPPO) C3 - 5th International Conference on Plant Proteomics T1 - Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation tolerance in Ramonda serbica Panc. UR - https://hdl.handle.net/21.15107/rcub_imagine_1850 ER -
@conference{ author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja", year = "2023", abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and fully restores its metabolic functions just one day after watering. The aim of this study was to identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance. We combined differential transcriptomics and proteomics with the analysis of phenolics, sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins and transcripts related to photosynthetic processes were downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate- glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall remodelling related to GLP-derived H2O2/HO● activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of important metabolic pathways during desiccation with the final aim to help improving the drought tolerance in crops.", publisher = "International Plant Proteomics Organization (INPPO)", journal = "5th International Conference on Plant Proteomics", title = "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation tolerance in Ramonda serbica Panc.", url = "https://hdl.handle.net/21.15107/rcub_imagine_1850" }
Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics International Plant Proteomics Organization (INPPO).. https://hdl.handle.net/21.15107/rcub_imagine_1850
Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics. 2023;. https://hdl.handle.net/21.15107/rcub_imagine_1850 .
Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation tolerance in Ramonda serbica Panc." in 5th International Conference on Plant Proteomics (2023), https://hdl.handle.net/21.15107/rcub_imagine_1850 .