Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment
Апстракт
About one in ten people contract a foodborne illness within a year. Children under the age of five are
the most affected, with 125,000 deaths each year. Many of the foodborne illness outbreaks can be
linked to the presence of biofilms in the food industry, and Salmonella enteritidis is an extremely
important foodborne pathogen that thrives in these conditions. It has been shown that biofilms can be
resistant to physical and chemical treatments used in cleaning and disinfection procedures in food
processing. The problem with using more aggressive disinfectants is that they often violate food safety
regulations. The use of enzymes which degrade biofilm matrix structural components should facilitate
current disinfection procedures and not compromise food safety. In this study, the anti-biofilm activity
of recombinantly expressed β-glucosidase B and its potential use as a protective agent to control
Salmonella biofilm formation is investigated. The putative target of this enzyme i...s cellulose, the
structural component of the Salmonella biofilm matrix. β-Glucosidase B deriving from the
environmental strain Microbacterium sp. BG28 was heterologously expressed in Escherichia coli and
successfully purified by affinity chromatography. The anti-biofilm activity of the enzyme was
evaluated in in vitro assays using various clinical isolates of S. enteritidis. The toxicity of the enzyme
was studied in Caenorhabditis elegans. β-glucosidase B effectively inhibited the formation of
Salmonella biofilms grown in a temperature range of 8°C to 37°C, achieving 50% inhibition at
concentrations of 100μg/ml. Biochemical characterization showed that the optimal pH activity of the
enzyme is between 6 and 7, with the highest activity observed at temperatures between 37°C and 47°C.
The absence of toxicity and other presented results indicate that beta-glucosidase B can be used in
biofilm control in the food industry.
Кључне речи:
Salmonella / biofilm / beta glucosidase / enzymeИзвор:
Genetics & Applications, 2023, 7, 2 (Special edition), 129-Издавач:
- Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo
Напомена:
- Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH May, 2023
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Atanasković, Marija AU - Morić, Ivana AU - Šenerović, Lidija PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1905 AB - About one in ten people contract a foodborne illness within a year. Children under the age of five are the most affected, with 125,000 deaths each year. Many of the foodborne illness outbreaks can be linked to the presence of biofilms in the food industry, and Salmonella enteritidis is an extremely important foodborne pathogen that thrives in these conditions. It has been shown that biofilms can be resistant to physical and chemical treatments used in cleaning and disinfection procedures in food processing. The problem with using more aggressive disinfectants is that they often violate food safety regulations. The use of enzymes which degrade biofilm matrix structural components should facilitate current disinfection procedures and not compromise food safety. In this study, the anti-biofilm activity of recombinantly expressed β-glucosidase B and its potential use as a protective agent to control Salmonella biofilm formation is investigated. The putative target of this enzyme is cellulose, the structural component of the Salmonella biofilm matrix. β-Glucosidase B deriving from the environmental strain Microbacterium sp. BG28 was heterologously expressed in Escherichia coli and successfully purified by affinity chromatography. The anti-biofilm activity of the enzyme was evaluated in in vitro assays using various clinical isolates of S. enteritidis. The toxicity of the enzyme was studied in Caenorhabditis elegans. β-glucosidase B effectively inhibited the formation of Salmonella biofilms grown in a temperature range of 8°C to 37°C, achieving 50% inhibition at concentrations of 100μg/ml. Biochemical characterization showed that the optimal pH activity of the enzyme is between 6 and 7, with the highest activity observed at temperatures between 37°C and 47°C. The absence of toxicity and other presented results indicate that beta-glucosidase B can be used in biofilm control in the food industry. PB - Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo C3 - Genetics & Applications T1 - Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment IS - 2 (Special edition) SP - 129 VL - 7 UR - https://hdl.handle.net/21.15107/rcub_imagine_1905 ER -
@conference{ author = "Atanasković, Marija and Morić, Ivana and Šenerović, Lidija", year = "2023", abstract = "About one in ten people contract a foodborne illness within a year. Children under the age of five are the most affected, with 125,000 deaths each year. Many of the foodborne illness outbreaks can be linked to the presence of biofilms in the food industry, and Salmonella enteritidis is an extremely important foodborne pathogen that thrives in these conditions. It has been shown that biofilms can be resistant to physical and chemical treatments used in cleaning and disinfection procedures in food processing. The problem with using more aggressive disinfectants is that they often violate food safety regulations. The use of enzymes which degrade biofilm matrix structural components should facilitate current disinfection procedures and not compromise food safety. In this study, the anti-biofilm activity of recombinantly expressed β-glucosidase B and its potential use as a protective agent to control Salmonella biofilm formation is investigated. The putative target of this enzyme is cellulose, the structural component of the Salmonella biofilm matrix. β-Glucosidase B deriving from the environmental strain Microbacterium sp. BG28 was heterologously expressed in Escherichia coli and successfully purified by affinity chromatography. The anti-biofilm activity of the enzyme was evaluated in in vitro assays using various clinical isolates of S. enteritidis. The toxicity of the enzyme was studied in Caenorhabditis elegans. β-glucosidase B effectively inhibited the formation of Salmonella biofilms grown in a temperature range of 8°C to 37°C, achieving 50% inhibition at concentrations of 100μg/ml. Biochemical characterization showed that the optimal pH activity of the enzyme is between 6 and 7, with the highest activity observed at temperatures between 37°C and 47°C. The absence of toxicity and other presented results indicate that beta-glucosidase B can be used in biofilm control in the food industry.", publisher = "Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo", journal = "Genetics & Applications", title = "Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment", number = "2 (Special edition)", pages = "129", volume = "7", url = "https://hdl.handle.net/21.15107/rcub_imagine_1905" }
Atanasković, M., Morić, I.,& Šenerović, L.. (2023). Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment. in Genetics & Applications Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 7(2 (Special edition)), 129. https://hdl.handle.net/21.15107/rcub_imagine_1905
Atanasković M, Morić I, Šenerović L. Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment. in Genetics & Applications. 2023;7(2 (Special edition)):129. https://hdl.handle.net/21.15107/rcub_imagine_1905 .
Atanasković, Marija, Morić, Ivana, Šenerović, Lidija, "Β-glucosidase b from microbacterium sp. Bg28 as a biofilm control agent In food processing environment" in Genetics & Applications, 7, no. 2 (Special edition) (2023):129, https://hdl.handle.net/21.15107/rcub_imagine_1905 .