Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression
Апстракт
To improve our understanding of the molecular mechanisms underlying seed priming, RNA
transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed
priming was performed by submerging the seeds in different priming agents for 24h at 4°C, followed
by rinsing with sterilized water and desiccation to the original moisture content. Silene sendtneri is a
species with no sequenced genome and annotation of de novo assembly of the transcriptome was done
using different species for the reference genome. Gene ontology (GO) analysis indicated that genes
related to heavy metal transporters and heat shock proteins are differentially expressed after priming in
relation to the used priming agent. Within these gene categories, genes such as heavy metal-associated
isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy
metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5,98) we...re
upregulated. Some gene categories are commonly highly expressed after seed priming regardless of the
used agent, but some specific gene categories have been recorded to be expressed in relation to the used
agent.
Кључне речи:
cd hyperaccumulator / heavy metal tolerance / seed priming / primed memoryИзвор:
Genetics & Applications, 2023, 7, 2 (Special edition), 143-Издавач:
- Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo
Напомена:
- Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH May, 2023
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Demir, Arnela AU - Selović, Alisa AU - Dahija, Sabina AU - Parić, Adisa AU - Samardžić, Jelena AU - Karalija, Erna PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1906 AB - To improve our understanding of the molecular mechanisms underlying seed priming, RNA transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed priming was performed by submerging the seeds in different priming agents for 24h at 4°C, followed by rinsing with sterilized water and desiccation to the original moisture content. Silene sendtneri is a species with no sequenced genome and annotation of de novo assembly of the transcriptome was done using different species for the reference genome. Gene ontology (GO) analysis indicated that genes related to heavy metal transporters and heat shock proteins are differentially expressed after priming in relation to the used priming agent. Within these gene categories, genes such as heavy metal-associated isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5,98) were upregulated. Some gene categories are commonly highly expressed after seed priming regardless of the used agent, but some specific gene categories have been recorded to be expressed in relation to the used agent. PB - Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo C3 - Genetics & Applications T1 - Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression IS - 2 (Special edition) SP - 143 VL - 7 UR - https://hdl.handle.net/21.15107/rcub_imagine_1906 ER -
@conference{ author = "Demir, Arnela and Selović, Alisa and Dahija, Sabina and Parić, Adisa and Samardžić, Jelena and Karalija, Erna", year = "2023", abstract = "To improve our understanding of the molecular mechanisms underlying seed priming, RNA transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed priming was performed by submerging the seeds in different priming agents for 24h at 4°C, followed by rinsing with sterilized water and desiccation to the original moisture content. Silene sendtneri is a species with no sequenced genome and annotation of de novo assembly of the transcriptome was done using different species for the reference genome. Gene ontology (GO) analysis indicated that genes related to heavy metal transporters and heat shock proteins are differentially expressed after priming in relation to the used priming agent. Within these gene categories, genes such as heavy metal-associated isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5,98) were upregulated. Some gene categories are commonly highly expressed after seed priming regardless of the used agent, but some specific gene categories have been recorded to be expressed in relation to the used agent.", publisher = "Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo", journal = "Genetics & Applications", title = "Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression", number = "2 (Special edition)", pages = "143", volume = "7", url = "https://hdl.handle.net/21.15107/rcub_imagine_1906" }
Demir, A., Selović, A., Dahija, S., Parić, A., Samardžić, J.,& Karalija, E.. (2023). Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression. in Genetics & Applications Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 7(2 (Special edition)), 143. https://hdl.handle.net/21.15107/rcub_imagine_1906
Demir A, Selović A, Dahija S, Parić A, Samardžić J, Karalija E. Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression. in Genetics & Applications. 2023;7(2 (Special edition)):143. https://hdl.handle.net/21.15107/rcub_imagine_1906 .
Demir, Arnela, Selović, Alisa, Dahija, Sabina, Parić, Adisa, Samardžić, Jelena, Karalija, Erna, "Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression" in Genetics & Applications, 7, no. 2 (Special edition) (2023):143, https://hdl.handle.net/21.15107/rcub_imagine_1906 .