Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers
Апстракт
Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for me...chanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].
Кључне речи:
biocatalysis / polyhydroxyalkanoates / biopolymers / enzyme engineering / depolymerase / BioICEPИзвор:
9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 2023, 9, 195-196Издавач:
- Beograd : Srpsko hemijsko društvo
Финансирање / пројекти:
- Present study was conducted as a part of Bio Innovation of a Circular Economy for Plastics – BioICEP.
- This project has received funding from the European Union's Horizon 2020 research and inovationprogramme under grant agreement No 870292
Напомена:
- KNJIGA IZVODA: 9. simpozijum Hemija i zaštita životne sredine Kladovo, 4-7. jun 2023. BOOK OF ABSTRACTS : 9th Symposium Chemistry and Environmental Protection Kladovo, 4-7th June 2023
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Marija, Nenadović AU - Pantelić, Brana AU - Ponjavić, Marijana AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1916 AB - Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics, owing to their biological origin, biodegradability, biocompatibility and structural diversity. However, environmental biodegradation of PHA is achieved in a time frame of several months to several years, depending on environmental conditions, and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into account the high production cost of PHA, landfilling at the end of life is not likely to be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ properties to create suitable biocatalysts for the industrially relevant time frame and operating conditions. In order to do so, we decided to randomize PhaZ sequences and functionally screen enzyme variants for accelerated PHA degradation and improved biocatalyst stability. Up to this day, various phaZ genes have been mutated solely for mechanistic purposes eg. Catalytic residue identification, and elucidation of the substrate recognition process [2,3,4,5,6,7]. PB - Beograd : Srpsko hemijsko društvo C3 - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023 T1 - Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers EP - 196 SP - 195 VL - 9 UR - https://hdl.handle.net/21.15107/rcub_imagine_1916 ER -
@conference{ author = "Marija, Nenadović and Pantelić, Brana and Ponjavić, Marijana and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics, owing to their biological origin, biodegradability, biocompatibility and structural diversity. However, environmental biodegradation of PHA is achieved in a time frame of several months to several years, depending on environmental conditions, and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into account the high production cost of PHA, landfilling at the end of life is not likely to be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ properties to create suitable biocatalysts for the industrially relevant time frame and operating conditions. In order to do so, we decided to randomize PhaZ sequences and functionally screen enzyme variants for accelerated PHA degradation and improved biocatalyst stability. Up to this day, various phaZ genes have been mutated solely for mechanistic purposes eg. Catalytic residue identification, and elucidation of the substrate recognition process [2,3,4,5,6,7].", publisher = "Beograd : Srpsko hemijsko društvo", journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023", title = "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers", pages = "196-195", volume = "9", url = "https://hdl.handle.net/21.15107/rcub_imagine_1916" }
Marija, N., Pantelić, B., Ponjavić, M.,& Nikodinović-Runić, J.. (2023). Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023 Beograd : Srpsko hemijsko društvo., 9, 195-196. https://hdl.handle.net/21.15107/rcub_imagine_1916
Marija N, Pantelić B, Ponjavić M, Nikodinović-Runić J. Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:195-196. https://hdl.handle.net/21.15107/rcub_imagine_1916 .
Marija, Nenadović, Pantelić, Brana, Ponjavić, Marijana, Nikodinović-Runić, Jasmina, "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):195-196, https://hdl.handle.net/21.15107/rcub_imagine_1916 .