DNA/BSA interactions and biological activity of prodigiosin and its copper(II) complex
Authors
Glišić, BiljanaAndrejević, Tina
Lazić, Jelena
Ilić-Tomić, Tatjana
Ašanin, Darko
Pantović, Bojana
Djuran, Miloš
Nikodinović-Runić, Jasmina
Conference object (Published version)
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Show full item recordAbstract
Prodigiosin (PG) is a red biopigment produced as a secondary metabolite by
microorganisms such as Serratia marcescens and Streptomyces. In recent years, this tripyrrole
compound has attracted an increasing attention due to its antibacterial, antimalarial, and
immunosuppressive activities [1]. It is also known for its antitumor activity, inducing the cell
death by apoptosis in different human cancer cell lines [2]. Considering this, in the present
study, we investigated the interactions of prodigiosin and its copper(II) complex (CuPG; the
structural formula is presented below), whose crystal structure was determined previously [2],
with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission
spectroscopy to clarify their binding affinities toward these biomolecules. The antimicrobial
activity of the synthesized CuPG complex and PG ligand was evaluated in vitro against various
microorganisms that can lead to many infections. Moreover, CuPG and PG were e...valuated in
a cell viability assay on a healthy MRC-5 cell line, as well as a panel of MDA-MB-231, A549,
A375, and HCT116 cancer cell lines.
Keywords:
prodigiosin / metal complex / interactions / binding / anticancerSource:
16th International Symposium on Applied Bioinorganic Chemistry, 2023, 264-Publisher:
- Greece : University of Ioannina
Funding / projects:
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-ScienceFundRS-Ideje-7730810)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200122 (University of Kragujevac, Faculty of Science) (RS-MESTD-inst-2020-200122)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200378 (Institute of Information Technology) (RS-MESTD-inst-2020-200378)
- Serbian Academy of Sciences and Arts under project No. F128
Note:
- Book of abstract:16th International Symposium on Applied Bioinorganic Chemistry (16-ISABC) 11-14th June University of Ioannina Greece
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Glišić, Biljana AU - Andrejević, Tina AU - Lazić, Jelena AU - Ilić-Tomić, Tatjana AU - Ašanin, Darko AU - Pantović, Bojana AU - Djuran, Miloš AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://isabc2023.com/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1926 AB - Prodigiosin (PG) is a red biopigment produced as a secondary metabolite by microorganisms such as Serratia marcescens and Streptomyces. In recent years, this tripyrrole compound has attracted an increasing attention due to its antibacterial, antimalarial, and immunosuppressive activities [1]. It is also known for its antitumor activity, inducing the cell death by apoptosis in different human cancer cell lines [2]. Considering this, in the present study, we investigated the interactions of prodigiosin and its copper(II) complex (CuPG; the structural formula is presented below), whose crystal structure was determined previously [2], with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy to clarify their binding affinities toward these biomolecules. The antimicrobial activity of the synthesized CuPG complex and PG ligand was evaluated in vitro against various microorganisms that can lead to many infections. Moreover, CuPG and PG were evaluated in a cell viability assay on a healthy MRC-5 cell line, as well as a panel of MDA-MB-231, A549, A375, and HCT116 cancer cell lines. PB - Greece : University of Ioannina C3 - 16th International Symposium on Applied Bioinorganic Chemistry T1 - DNA/BSA interactions and biological activity of prodigiosin and its copper(II) complex SP - 264 UR - https://hdl.handle.net/21.15107/rcub_imagine_1926 ER -
@conference{ author = "Glišić, Biljana and Andrejević, Tina and Lazić, Jelena and Ilić-Tomić, Tatjana and Ašanin, Darko and Pantović, Bojana and Djuran, Miloš and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Prodigiosin (PG) is a red biopigment produced as a secondary metabolite by microorganisms such as Serratia marcescens and Streptomyces. In recent years, this tripyrrole compound has attracted an increasing attention due to its antibacterial, antimalarial, and immunosuppressive activities [1]. It is also known for its antitumor activity, inducing the cell death by apoptosis in different human cancer cell lines [2]. Considering this, in the present study, we investigated the interactions of prodigiosin and its copper(II) complex (CuPG; the structural formula is presented below), whose crystal structure was determined previously [2], with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy to clarify their binding affinities toward these biomolecules. The antimicrobial activity of the synthesized CuPG complex and PG ligand was evaluated in vitro against various microorganisms that can lead to many infections. Moreover, CuPG and PG were evaluated in a cell viability assay on a healthy MRC-5 cell line, as well as a panel of MDA-MB-231, A549, A375, and HCT116 cancer cell lines.", publisher = "Greece : University of Ioannina", journal = "16th International Symposium on Applied Bioinorganic Chemistry", title = "DNA/BSA interactions and biological activity of prodigiosin and its copper(II) complex", pages = "264", url = "https://hdl.handle.net/21.15107/rcub_imagine_1926" }
Glišić, B., Andrejević, T., Lazić, J., Ilić-Tomić, T., Ašanin, D., Pantović, B., Djuran, M.,& Nikodinović-Runić, J.. (2023). DNA/BSA interactions and biological activity of prodigiosin and its copper(II) complex. in 16th International Symposium on Applied Bioinorganic Chemistry Greece : University of Ioannina., 264. https://hdl.handle.net/21.15107/rcub_imagine_1926
Glišić B, Andrejević T, Lazić J, Ilić-Tomić T, Ašanin D, Pantović B, Djuran M, Nikodinović-Runić J. DNA/BSA interactions and biological activity of prodigiosin and its copper(II) complex. in 16th International Symposium on Applied Bioinorganic Chemistry. 2023;:264. https://hdl.handle.net/21.15107/rcub_imagine_1926 .
Glišić, Biljana, Andrejević, Tina, Lazić, Jelena, Ilić-Tomić, Tatjana, Ašanin, Darko, Pantović, Bojana, Djuran, Miloš, Nikodinović-Runić, Jasmina, "DNA/BSA interactions and biological activity of prodigiosin and its copper(II) complex" in 16th International Symposium on Applied Bioinorganic Chemistry (2023):264, https://hdl.handle.net/21.15107/rcub_imagine_1926 .