Stereo-seq: Large Field of View-Spatially Resolved Transcriptomics at Nanoscale Resolution
Конференцијски прилог (Објављена верзија)
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© 2023 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
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STOmics’ Stereo-seq technology is a world-leading unbiased whole transcriptome spatial
omics platform that combines nanoscale resolution with the centimeter-level field of
view. The Stereo-seq chip comprises billions of probes, each with spatial barcodes, in a
patterned array. Biological tissue is sectioned and loaded to the Stereo-seq chip, followed
by fixation and permeabilization. Then mRNA molecules from the tissue section hybridize
with the barcoded probes, and reverse transcription produces spatially barcoded cDNA.
Following library preparation and sequencing to generate spatially resolved Stereo-seq
data from the tissue section. Proprietary cloud-based analysis reconstitutes the data
and enables visualization of the transcriptomics of the original tissue section in space,
empowering further research and assembly of spatial omics atlases.
Кључне речи:
single-cell unbiased whole transcriptomics / spatial omics / nanoscale resolution / centimeter-level field of viewИзвор:
4th Belgrade Bioinformatics Conference, 2023, 4, 10-10Издавач:
- Belgrade : Institute of molecular genetics and genetic engineering
Напомена:
- Book of abstract: 4th Belgrade Bioinformatics Conference, June 19-23, 2023
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Batista Perez, Javier PY - 2023 UR - https://belbi.bg.ac.rs/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1945 AB - STOmics’ Stereo-seq technology is a world-leading unbiased whole transcriptome spatial omics platform that combines nanoscale resolution with the centimeter-level field of view. The Stereo-seq chip comprises billions of probes, each with spatial barcodes, in a patterned array. Biological tissue is sectioned and loaded to the Stereo-seq chip, followed by fixation and permeabilization. Then mRNA molecules from the tissue section hybridize with the barcoded probes, and reverse transcription produces spatially barcoded cDNA. Following library preparation and sequencing to generate spatially resolved Stereo-seq data from the tissue section. Proprietary cloud-based analysis reconstitutes the data and enables visualization of the transcriptomics of the original tissue section in space, empowering further research and assembly of spatial omics atlases. PB - Belgrade : Institute of molecular genetics and genetic engineering C3 - 4th Belgrade Bioinformatics Conference T1 - Stereo-seq: Large Field of View-Spatially Resolved Transcriptomics at Nanoscale Resolution EP - 10 SP - 10 VL - 4 UR - https://hdl.handle.net/21.15107/rcub_imagine_1945 ER -
@conference{ author = "Batista Perez, Javier", year = "2023", abstract = "STOmics’ Stereo-seq technology is a world-leading unbiased whole transcriptome spatial omics platform that combines nanoscale resolution with the centimeter-level field of view. The Stereo-seq chip comprises billions of probes, each with spatial barcodes, in a patterned array. Biological tissue is sectioned and loaded to the Stereo-seq chip, followed by fixation and permeabilization. Then mRNA molecules from the tissue section hybridize with the barcoded probes, and reverse transcription produces spatially barcoded cDNA. Following library preparation and sequencing to generate spatially resolved Stereo-seq data from the tissue section. Proprietary cloud-based analysis reconstitutes the data and enables visualization of the transcriptomics of the original tissue section in space, empowering further research and assembly of spatial omics atlases.", publisher = "Belgrade : Institute of molecular genetics and genetic engineering", journal = "4th Belgrade Bioinformatics Conference", title = "Stereo-seq: Large Field of View-Spatially Resolved Transcriptomics at Nanoscale Resolution", pages = "10-10", volume = "4", url = "https://hdl.handle.net/21.15107/rcub_imagine_1945" }
Batista Perez, J.. (2023). Stereo-seq: Large Field of View-Spatially Resolved Transcriptomics at Nanoscale Resolution. in 4th Belgrade Bioinformatics Conference Belgrade : Institute of molecular genetics and genetic engineering., 4, 10-10. https://hdl.handle.net/21.15107/rcub_imagine_1945
Batista Perez J. Stereo-seq: Large Field of View-Spatially Resolved Transcriptomics at Nanoscale Resolution. in 4th Belgrade Bioinformatics Conference. 2023;4:10-10. https://hdl.handle.net/21.15107/rcub_imagine_1945 .
Batista Perez, Javier, "Stereo-seq: Large Field of View-Spatially Resolved Transcriptomics at Nanoscale Resolution" in 4th Belgrade Bioinformatics Conference, 4 (2023):10-10, https://hdl.handle.net/21.15107/rcub_imagine_1945 .