Transcriptome analysis of Atdss1 mutants in response to oxidative stress
Апстракт
DSS1 (deletion of split hand/split foot 1) is a highly conserved, eukaryotic, and multifunctional
protein. DSS1 as a small intrinsically disordered protein binds to multiple proteins when it
gains a final conformation. There are two highly homologous genes, DSS1(I) and DSS1(V) in the
Arabidopsis genome. Our aim is to examine Atdss1 mutants through oxidative stress. We
obtained separate stable lines of Arabidopsis containing mutations in DSS1s using
CRISPR/Cas9 technology. After H2O2 treatment, mutant seedlings showed increased
sensitivity to oxidative stress in comparison to WT plants. Transcriptome analysis showed
that dss1(I)del25 and dss1(V)ins18 mutations caused 2762 and 2335 differentially expressed
genes compared to WT under oxidative stress, respectively. We found that upregulated
expression was in genes involved in homologue recombination and RNA transport in both dss1
lines. The most downregulated genes are classified into flavonoid biosynthesis and MAPK
signaling p...athway.
Кључне речи:
AtDSS1 / CRISPR / transcriptome / oxidative stressИзвор:
14th International Conference of the French Society of Plant Biology, 2023, 14, 172-172Издавач:
- Plant Biology Europe
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Nikolić, Ivana AU - Timotijević, Gordana AU - Samardžić, Jelena AU - Milisavljević, Mira PY - 2023 UR - https://europlantbiology2023.org/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1972 AB - DSS1 (deletion of split hand/split foot 1) is a highly conserved, eukaryotic, and multifunctional protein. DSS1 as a small intrinsically disordered protein binds to multiple proteins when it gains a final conformation. There are two highly homologous genes, DSS1(I) and DSS1(V) in the Arabidopsis genome. Our aim is to examine Atdss1 mutants through oxidative stress. We obtained separate stable lines of Arabidopsis containing mutations in DSS1s using CRISPR/Cas9 technology. After H2O2 treatment, mutant seedlings showed increased sensitivity to oxidative stress in comparison to WT plants. Transcriptome analysis showed that dss1(I)del25 and dss1(V)ins18 mutations caused 2762 and 2335 differentially expressed genes compared to WT under oxidative stress, respectively. We found that upregulated expression was in genes involved in homologue recombination and RNA transport in both dss1 lines. The most downregulated genes are classified into flavonoid biosynthesis and MAPK signaling pathway. PB - Plant Biology Europe C3 - 14th International Conference of the French Society of Plant Biology T1 - Transcriptome analysis of Atdss1 mutants in response to oxidative stress EP - 172 SP - 172 VL - 14 UR - https://hdl.handle.net/21.15107/rcub_imagine_1972 ER -
@conference{ author = "Nikolić, Ivana and Timotijević, Gordana and Samardžić, Jelena and Milisavljević, Mira", year = "2023", abstract = "DSS1 (deletion of split hand/split foot 1) is a highly conserved, eukaryotic, and multifunctional protein. DSS1 as a small intrinsically disordered protein binds to multiple proteins when it gains a final conformation. There are two highly homologous genes, DSS1(I) and DSS1(V) in the Arabidopsis genome. Our aim is to examine Atdss1 mutants through oxidative stress. We obtained separate stable lines of Arabidopsis containing mutations in DSS1s using CRISPR/Cas9 technology. After H2O2 treatment, mutant seedlings showed increased sensitivity to oxidative stress in comparison to WT plants. Transcriptome analysis showed that dss1(I)del25 and dss1(V)ins18 mutations caused 2762 and 2335 differentially expressed genes compared to WT under oxidative stress, respectively. We found that upregulated expression was in genes involved in homologue recombination and RNA transport in both dss1 lines. The most downregulated genes are classified into flavonoid biosynthesis and MAPK signaling pathway.", publisher = "Plant Biology Europe", journal = "14th International Conference of the French Society of Plant Biology", title = "Transcriptome analysis of Atdss1 mutants in response to oxidative stress", pages = "172-172", volume = "14", url = "https://hdl.handle.net/21.15107/rcub_imagine_1972" }
Nikolić, I., Timotijević, G., Samardžić, J.,& Milisavljević, M.. (2023). Transcriptome analysis of Atdss1 mutants in response to oxidative stress. in 14th International Conference of the French Society of Plant Biology Plant Biology Europe., 14, 172-172. https://hdl.handle.net/21.15107/rcub_imagine_1972
Nikolić I, Timotijević G, Samardžić J, Milisavljević M. Transcriptome analysis of Atdss1 mutants in response to oxidative stress. in 14th International Conference of the French Society of Plant Biology. 2023;14:172-172. https://hdl.handle.net/21.15107/rcub_imagine_1972 .
Nikolić, Ivana, Timotijević, Gordana, Samardžić, Jelena, Milisavljević, Mira, "Transcriptome analysis of Atdss1 mutants in response to oxidative stress" in 14th International Conference of the French Society of Plant Biology, 14 (2023):172-172, https://hdl.handle.net/21.15107/rcub_imagine_1972 .