Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity
Аутори
Trivanović, DrenkaKovačević, Jovana
Arsić, Aleksandra
Vujačić, Marko
Bogosavljević, Nikola
Okić Djordjević, Ivana
Živanović, Milena
Mojsilović, Slavko
Maljković, Mirjana
Jauković, Aleksandra
Остала ауторства
Morić, IvanaĐorđević, Valentina
Конференцијски прилог (Објављена верзија)
,
© 2023 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт
As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated
skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT).
Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT)
were obtained from matched osteoarthritis patients. Patients were classified into two
groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored
by using thin layer/gas chromatography coupled with cellular and molecular assays.
Data were interpreted and visualized by applying linear discriminant analysis (LDA) and
hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric
tests and Spearman’s rank correlation.
Analyses of total lipids revealed significantly reduced triglyceride content in femoral
(fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously
released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMA...T than
in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that
fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in
FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid
was increased in aged group in all AT depots, while stearic acid declined in aged group in
BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037).
Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells
was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot.
Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their
acetabular counterparts.
Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment
of stem cells within this skeletal site.
Кључне речи:
bone marrow adipose tissue / fatty acids / stem cells / adipogenesis / agingИзвор:
4th Belgrade Bioinformatics Conference, 2023, 4, 54-54Издавач:
- Belgrade : Institute of molecular genetics and genetic engineering
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-MESTD-inst-2020-200015)
Напомена:
- Book of abstract: 4th Belgrade Bioinformatics Conference, June 19-23, 2023
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Trivanović, Drenka AU - Kovačević, Jovana AU - Arsić, Aleksandra AU - Vujačić, Marko AU - Bogosavljević, Nikola AU - Okić Djordjević, Ivana AU - Živanović, Milena AU - Mojsilović, Slavko AU - Maljković, Mirjana AU - Jauković, Aleksandra PY - 2023 UR - https://belbi.bg.ac.rs/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1995 AB - As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT). Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT) were obtained from matched osteoarthritis patients. Patients were classified into two groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored by using thin layer/gas chromatography coupled with cellular and molecular assays. Data were interpreted and visualized by applying linear discriminant analysis (LDA) and hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric tests and Spearman’s rank correlation. Analyses of total lipids revealed significantly reduced triglyceride content in femoral (fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid was increased in aged group in all AT depots, while stearic acid declined in aged group in BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037). Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot. Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their acetabular counterparts. Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment of stem cells within this skeletal site. PB - Belgrade : Institute of molecular genetics and genetic engineering C3 - 4th Belgrade Bioinformatics Conference T1 - Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity EP - 54 SP - 54 VL - 4 UR - https://hdl.handle.net/21.15107/rcub_imagine_1995 ER -
@conference{ author = "Trivanović, Drenka and Kovačević, Jovana and Arsić, Aleksandra and Vujačić, Marko and Bogosavljević, Nikola and Okić Djordjević, Ivana and Živanović, Milena and Mojsilović, Slavko and Maljković, Mirjana and Jauković, Aleksandra", year = "2023", abstract = "As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT). Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT) were obtained from matched osteoarthritis patients. Patients were classified into two groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored by using thin layer/gas chromatography coupled with cellular and molecular assays. Data were interpreted and visualized by applying linear discriminant analysis (LDA) and hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric tests and Spearman’s rank correlation. Analyses of total lipids revealed significantly reduced triglyceride content in femoral (fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid was increased in aged group in all AT depots, while stearic acid declined in aged group in BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037). Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot. Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their acetabular counterparts. Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment of stem cells within this skeletal site.", publisher = "Belgrade : Institute of molecular genetics and genetic engineering", journal = "4th Belgrade Bioinformatics Conference", title = "Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity", pages = "54-54", volume = "4", url = "https://hdl.handle.net/21.15107/rcub_imagine_1995" }
Trivanović, D., Kovačević, J., Arsić, A., Vujačić, M., Bogosavljević, N., Okić Djordjević, I., Živanović, M., Mojsilović, S., Maljković, M.,& Jauković, A.. (2023). Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity. in 4th Belgrade Bioinformatics Conference Belgrade : Institute of molecular genetics and genetic engineering., 4, 54-54. https://hdl.handle.net/21.15107/rcub_imagine_1995
Trivanović D, Kovačević J, Arsić A, Vujačić M, Bogosavljević N, Okić Djordjević I, Živanović M, Mojsilović S, Maljković M, Jauković A. Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity. in 4th Belgrade Bioinformatics Conference. 2023;4:54-54. https://hdl.handle.net/21.15107/rcub_imagine_1995 .
Trivanović, Drenka, Kovačević, Jovana, Arsić, Aleksandra, Vujačić, Marko, Bogosavljević, Nikola, Okić Djordjević, Ivana, Živanović, Milena, Mojsilović, Slavko, Maljković, Mirjana, Jauković, Aleksandra, "Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity" in 4th Belgrade Bioinformatics Conference, 4 (2023):54-54, https://hdl.handle.net/21.15107/rcub_imagine_1995 .