Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation
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© 2023 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
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Insects are the most diverse group in the animal kingdom, accounting for about two-thirds
of all animals. Cytochrome c oxidase subunit 1 (COI) is the most commonly used marker
gene for animal species delineation. However, the accuracy of this approach crucially
depends on the degree of overlap between the intra- and interspecific variations.
Recently, we have identified a new species, Heterogynis serbica sp. n. (Lepidoptera:
Zygaenoidea, Heterogynidae) found on the Mt. Kopaonik, Republic of Serbia, Balkan
Peninsula. This was done by integrating taxonomic approach using morpho-anatomical
characteristics by comparative scanning electron microscopy (SEM), linear wing
morphometry and COI-based DNA barcoding [1]. In this study, we have used a set of
bioinformatics tools available online, to determine the differences in secondary and
tertiary structure of the COI proteins from H. serbica sp. n. and H. zikici. We also compared
the amino acid distribution and COI motif profiles betw...een the two species. Our results
provide strong evidence that protein structure of COI can help with COI-based DNA
barcoding for taxon-specific purposes of species identification and delimitation studies.
Millions of COI DNA sequences deposited in the public domain (which are still growing)
carry huge potential for a comprehensive assessment of genetic variation in COI among
insects by using here described analysis.
Кључне речи:
cytochrome c oxidase subunit 1 / Heterogynidae / Heterogynis sp. / Lepidoptera, conserved protein motif / protein sequence / secondary and tertiary protein structure / transmembrane helicesИзвор:
4th Belgrade Bioinformatics Conference, 2023, 4, 109-109Издавач:
- Belgrade : Institute of molecular genetics and genetic engineering
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200197 (Институт за низијско шумарство и животну средину, Нови Сад) (RS-MESTD-inst-2020-200197)
Напомена:
- Book of abstract: 4th Belgrade Bioinformatics Conference, June 19-23, 2023
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Vidović, Marija AU - Galović, Vladislava PY - 2023 UR - https://belbi.bg.ac.rs/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2054 AB - Insects are the most diverse group in the animal kingdom, accounting for about two-thirds of all animals. Cytochrome c oxidase subunit 1 (COI) is the most commonly used marker gene for animal species delineation. However, the accuracy of this approach crucially depends on the degree of overlap between the intra- and interspecific variations. Recently, we have identified a new species, Heterogynis serbica sp. n. (Lepidoptera: Zygaenoidea, Heterogynidae) found on the Mt. Kopaonik, Republic of Serbia, Balkan Peninsula. This was done by integrating taxonomic approach using morpho-anatomical characteristics by comparative scanning electron microscopy (SEM), linear wing morphometry and COI-based DNA barcoding [1]. In this study, we have used a set of bioinformatics tools available online, to determine the differences in secondary and tertiary structure of the COI proteins from H. serbica sp. n. and H. zikici. We also compared the amino acid distribution and COI motif profiles between the two species. Our results provide strong evidence that protein structure of COI can help with COI-based DNA barcoding for taxon-specific purposes of species identification and delimitation studies. Millions of COI DNA sequences deposited in the public domain (which are still growing) carry huge potential for a comprehensive assessment of genetic variation in COI among insects by using here described analysis. PB - Belgrade : Institute of molecular genetics and genetic engineering C3 - 4th Belgrade Bioinformatics Conference T1 - Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation EP - 109 SP - 109 VL - 4 UR - https://hdl.handle.net/21.15107/rcub_imagine_2054 ER -
@conference{ author = "Vidović, Marija and Galović, Vladislava", year = "2023", abstract = "Insects are the most diverse group in the animal kingdom, accounting for about two-thirds of all animals. Cytochrome c oxidase subunit 1 (COI) is the most commonly used marker gene for animal species delineation. However, the accuracy of this approach crucially depends on the degree of overlap between the intra- and interspecific variations. Recently, we have identified a new species, Heterogynis serbica sp. n. (Lepidoptera: Zygaenoidea, Heterogynidae) found on the Mt. Kopaonik, Republic of Serbia, Balkan Peninsula. This was done by integrating taxonomic approach using morpho-anatomical characteristics by comparative scanning electron microscopy (SEM), linear wing morphometry and COI-based DNA barcoding [1]. In this study, we have used a set of bioinformatics tools available online, to determine the differences in secondary and tertiary structure of the COI proteins from H. serbica sp. n. and H. zikici. We also compared the amino acid distribution and COI motif profiles between the two species. Our results provide strong evidence that protein structure of COI can help with COI-based DNA barcoding for taxon-specific purposes of species identification and delimitation studies. Millions of COI DNA sequences deposited in the public domain (which are still growing) carry huge potential for a comprehensive assessment of genetic variation in COI among insects by using here described analysis.", publisher = "Belgrade : Institute of molecular genetics and genetic engineering", journal = "4th Belgrade Bioinformatics Conference", title = "Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation", pages = "109-109", volume = "4", url = "https://hdl.handle.net/21.15107/rcub_imagine_2054" }
Vidović, M.,& Galović, V.. (2023). Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation. in 4th Belgrade Bioinformatics Conference Belgrade : Institute of molecular genetics and genetic engineering., 4, 109-109. https://hdl.handle.net/21.15107/rcub_imagine_2054
Vidović M, Galović V. Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation. in 4th Belgrade Bioinformatics Conference. 2023;4:109-109. https://hdl.handle.net/21.15107/rcub_imagine_2054 .
Vidović, Marija, Galović, Vladislava, "Protein structural differences in Cytochrome c oxidase subunit 1 of two Heterogynis species as a new approach for species delimitation" in 4th Belgrade Bioinformatics Conference, 4 (2023):109-109, https://hdl.handle.net/21.15107/rcub_imagine_2054 .