Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine
Samo za registrovane korisnike
2023
Autori
Pantelić, LenaBogojević Škaro, Sanja
Vojnović, Sandra
Oliveira, Rui
Lazić, Jelena
Ilić-Tomić, Tatjana
Milivojević, Dušan
Nikodinović-Runić, Jasmina
Članak u časopisu (Recenzirana verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermen...ter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines.
Ključne reči:
1-hydroxyphenazine / bacterial pigment / biorefinery / food waste valorization / pyocyaninIzvor:
Enzyme and Microbial Technology, 2023, 171, 110322-Finansiranje / projekti:
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-ScienceFundRS-Ideje-7730810)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
- This work was also supported by the Associate Laboratory for Green Chemistry – LAQV 490 (https://laqv.requimte.pt/) financed by FCT/MCTES (UIDB/50006/2020)
URI
https://www.sciencedirect.com/science/article/pii/S0141022923001308https://imagine.imgge.bg.ac.rs/handle/123456789/2078
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Pantelić, Lena AU - Bogojević Škaro, Sanja AU - Vojnović, Sandra AU - Oliveira, Rui AU - Lazić, Jelena AU - Ilić-Tomić, Tatjana AU - Milivojević, Dušan AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://www.sciencedirect.com/science/article/pii/S0141022923001308 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2078 AB - Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermenter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines. T2 - Enzyme and Microbial Technology T1 - Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine SP - 110322 VL - 171 DO - 10.1016/j.enzmictec.2023.110322 ER -
@article{ author = "Pantelić, Lena and Bogojević Škaro, Sanja and Vojnović, Sandra and Oliveira, Rui and Lazić, Jelena and Ilić-Tomić, Tatjana and Milivojević, Dušan and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermenter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines.", journal = "Enzyme and Microbial Technology", title = "Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine", pages = "110322", volume = "171", doi = "10.1016/j.enzmictec.2023.110322" }
Pantelić, L., Bogojević Škaro, S., Vojnović, S., Oliveira, R., Lazić, J., Ilić-Tomić, T., Milivojević, D.,& Nikodinović-Runić, J.. (2023). Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine. in Enzyme and Microbial Technology, 171, 110322. https://doi.org/10.1016/j.enzmictec.2023.110322
Pantelić L, Bogojević Škaro S, Vojnović S, Oliveira R, Lazić J, Ilić-Tomić T, Milivojević D, Nikodinović-Runić J. Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine. in Enzyme and Microbial Technology. 2023;171:110322. doi:10.1016/j.enzmictec.2023.110322 .
Pantelić, Lena, Bogojević Škaro, Sanja, Vojnović, Sandra, Oliveira, Rui, Lazić, Jelena, Ilić-Tomić, Tatjana, Milivojević, Dušan, Nikodinović-Runić, Jasmina, "Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine" in Enzyme and Microbial Technology, 171 (2023):110322, https://doi.org/10.1016/j.enzmictec.2023.110322 . .